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A novel heterologous expression strategy for the quorum-quenching enzyme MomL in Lysobacter enzymogenes to the inhibit pathogenicity of Pectobacterium.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • الموضوع:
      Carboxylic Ester Hydrolases/*metabolism ; Lysobacter/*enzymology ; Lysobacter/*metabolism ; Pectobacterium carotovorum/*pathogenicity ; Plant Diseases/*prevention & control ; Quorum Sensing/*physiology; Carboxylic Ester Hydrolases/genetics ; Gene Expression Regulation, Bacterial ; Lysobacter/genetics ; Virulence/physiology ; Virulence Factors/metabolism
    • نبذة مختصرة :
      Quorum-quenching (QQ) enzymes can block the quorum-sensing (QS) system and prevent the expression of QS-controlled pathogenic factors in bacteria. However, the low expression levels of QQ proteins in the original host bacteria have affected their widespread application. In this study, we heterologously expressed momL, encoding a QQ enzyme with high activity, in Lysobacter enzymogenes. A "yellow-to-white" selection marker and the high-constitutive-expression promoter P groEL were used in this novel heterologous expression system. In addition, we optimized the spacer between the SD sequence and the initiator to improve the efficiency of the expression system by 1.54-fold. The engineered strain LeMomL degraded the AHL molecule and the virulence factors of Pectobacterium carotovorum subsp. carotovora (Pcc). Additionally, LeMomL significantly decreased the disease caused by Pcc in Chinese cabbages and carrot root tissues. In conclusion, this novel and facile L. enzymogenes expression strategy has good prospects and is an ideal approach for foreign protein expression.
    • Grant Information:
      31870023, 31571970, 41506160 National Natural Science Foundation of China; YESS20160009 Young Elite Scientists Sponsorship Program by CAST
    • Contributed Indexing:
      Keywords: LeMomL; MomL; Pathogenic; Quorum sensing; Transformant
    • الرقم المعرف:
      0 (Virulence Factors)
      EC 3.1.1.- (Carboxylic Ester Hydrolases)
      EC 3.1.1.- (N-acyl homoserine lactonase)
    • الموضوع:
      Lysobacter enzymogenes
    • الموضوع:
      Date Created: 20191029 Date Completed: 20200309 Latest Revision: 20200309
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1007/s00253-019-10166-w
    • الرقم المعرف:
      31656979