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Redirecting the lipid metabolism of the yeast Starmerella bombicola from glycolipid to fatty acid production.

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  • معلومة اضافية
    • المصدر:
      Publisher: Oxford University Press Country of Publication: Germany NLM ID: 9705544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5535 (Electronic) Linking ISSN: 13675435 NLM ISO Abbreviation: J Ind Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2021- : [Oxford] : Oxford University Press
      Original Publication: Houndmills, Basingstoke, Hampshire, UK : Published by Stockton Press on behalf of the Society for Industrial Microbiology, c1996-
    • الموضوع:
      Fatty Acids/*biosynthesis ; Glycolipids/*metabolism ; Saccharomycetales/*metabolism; Glycolipids/chemistry ; Lipid Metabolism ; Metabolic Engineering ; Oxidation-Reduction ; Saccharomycetales/genetics
    • نبذة مختصرة :
      Free fatty acids are basic oleochemicals implemented in a range of applications including surfactants, lubricants, paints, plastics, and cosmetics. Microbial fatty acid biosynthesis has gained much attention as it provides a sustainable alternative for petrol- and plant oil-derived chemicals. The yeast Starmerella bombicola is a microbial cell factory that naturally employs its powerful lipid metabolism for the production of the biodetergents sophorolipids (> 300 g/L). However, in this study we exploit the lipidic potential of S. bombicola and convert it from the glycolipid production platform into a free fatty acid cell factory. We used several metabolic engineering strategies to promote extracellular fatty acid accumulation which include blocking competing pathways (sophorolipid biosynthesis and β-oxidation) and preventing free fatty acid activation. The best producing mutant (Δcyp52m1Δfaa1Δmfe2) secreted 0.933 g/L (± 0.04) free fatty acids with a majority of C18:1 (43.8%) followed by C18:0 and C16:0 (40.0 and 13.2%, respectively). Interestingly, deletion of SbFaa1 in a strain still producing sophorolipids also resulted in 25% increased de novo sophorolipid synthesis (P = 0.0089) and when oil was supplemented to the same strain, a 50% increase in sophorolipid production was observed compared to the wild type (P = 0.03). We believe that our work is pivotal for the further development and exploration of S. bombicola as a platform for synthesis of environmentally friendly oleochemicals.
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    • Grant Information:
      151610 Fonds Wetenschappelijk Onderzoek
    • Contributed Indexing:
      Keywords: Free fatty acid; Lipid metabolism; Sophorolipid; Starmerella bombicola; Yeast
    • الرقم المعرف:
      0 (Fatty Acids)
      0 (Glycolipids)
    • الموضوع:
      Date Created: 20190913 Date Completed: 20200124 Latest Revision: 20200124
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1007/s10295-019-02234-x
    • الرقم المعرف:
      31512095