Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice ( Oryza sativa L.).

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Antioxidants* ; Genes, Plant* ; Quantitative Trait, Heritable*; Oryza/*genetics ; Seeds/*genetics; Lipid Peroxidation/genetics ; Mixed Function Oxygenases/genetics ; Mixed Function Oxygenases/metabolism ; Oryza/metabolism ; Polymorphism, Single Nucleotide ; Seeds/metabolism

Seed storability, defined as the ability to remain alive during storage, is an important agronomic and physiological characteristic, but the underlying genetic mechanism remains largely unclear. Here, we report quantitative trait loci (QTLs) analyses for seed storability using a high-density single nucleotide polymorphism linkage map in the backcross recombinant inbred lines that was derived from a cross of a japonica cultivar, Nipponbare, and an indica cultivar, 9311. Seven putative QTLs were identified for seed storability under natural storage, each explaining 3.6-9.0% of the phenotypic variation in this population. Among these QTLs, qSS1 with the 9311 alleles promoting seed storability was further validated in near-isogenic line and its derived-F 2 population. The other locus ( qSS3.1 ) for seed storability colocalized with a locus for germination ability under hydrogen peroxide, which is recognized as an oxidant molecule that causes lipid damage. Transgenic experiments validated that a candidate gene ( OsFAH2 ) resides the qSS3.1 region controlling seed storability and antioxidant capability. Overexpression of OsFAH2 that encodes a fatty acid hydroxylase reduced lipid preoxidation and increased seed storability. These findings provide new insights into the genetic and physiological bases of seed storability and will be useful for the improvement of seed storability in rice.
Competing Interests: The authors declare no conflicts of interest.

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31671656, 31971864 National Natural Science Foundation of China; 2662018YJ025, 2662016QD040 Fundamental Research Funds for the Central Universities; 201710504001 National Student's Platform for Innovation and Entrepreneurship Training Program

Keywords: fatty acid hydroxylase; genotyping by sequencing; oxidative stress; quantitative trait locus; rice; seed viability

0 (Antioxidants)
EC 1.- (Mixed Function Oxygenases)

Date Created: 20190912 Date Completed: 20200130 Latest Revision: 20200130

20240829

PMC6770242

10.3390/ijms20184442

31505900