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High-performance liquid chromatography and Enzyme-Linked Immunosorbent Assay techniques for detection and quantification of aflatoxin B 1 in feed samples: a comparative study.

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  • معلومة اضافية
    • المصدر:
      Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500 (Electronic) Linking ISSN: 17560500 NLM ISO Abbreviation: BMC Res Notes Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Biomed Central, 2008.
    • الموضوع:
      Aflatoxin B1/*isolation & purification ; Chromatography, High Pressure Liquid/*standards ; Enzyme-Linked Immunosorbent Assay/*standards ; Food Contamination/*analysis ; Zea mays/*chemistry; Acetonitriles/chemistry ; Animal Feed/analysis ; Animals ; Chromatography, High Pressure Liquid/methods ; Enzyme-Linked Immunosorbent Assay/methods ; Ethanol/chemistry ; Humans ; Reproducibility of Results ; Sensitivity and Specificity ; Solid Phase Extraction/methods ; Solvents/chemistry ; Water/chemistry
    • نبذة مختصرة :
      Objective: Comparison was done between high-performance liquid chromatography (HPLC) and a competitive enzyme-linked immunosorbent assay (ELISA) for detection and quantification of aflatoxin B 1 (AFB 1 ) in feed samples. The two procedures were standardized and validated before the actual experiment. Five concentrations (0, 5, 10, 20 and 30 ppb) of feed samples were used for both methods. For the HPLC technique, the samples were extracted in acetonitrile/water (90/10) solution, cleaned-up using solid phase extraction (SPE) column, and derivatized by water/trifluoroacetic acid/glacial acetic acid (35/10/5) solution before instrument analysis. The samples were extracted in 70% methanol for the ELISA technique.
      Results: The two tests showed very strong linearity with correlation coefficient value of > 0.99 using standard solutions. The mean recovery rate was 92.42% (with relative standard deviation (RSD) of 5.97) and 75.64% (RSD = 34.88) for HPLC and ELISA, respectively. There was no statistically significant difference in recovery rate between the two methods. There was a positive correlation (r = 0.84) between them which indicated that the two techniques can be used to detect and quantify aflatoxin B 1 in feed samples. However, there were variations among replicates for the ELISA method, which shows that this method is more applicable for screening purposes.
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    • Contributed Indexing:
      Keywords: Aflatoxin B1; ELISA; Feed; HPLC
    • الرقم المعرف:
      0 (Acetonitriles)
      0 (Solvents)
      059QF0KO0R (Water)
      3K9958V90M (Ethanol)
      9N2N2Y55MH (Aflatoxin B1)
      Z072SB282N (acetonitrile)
    • الموضوع:
      Date Created: 20190809 Date Completed: 20200113 Latest Revision: 20200225
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC6686514
    • الرقم المعرف:
      10.1186/s13104-019-4538-z
    • الرقم المعرف:
      31391088