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Cell surface display of proteins on filamentous fungi.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin ; New York : Springer International, c1984-
    • الموضوع:
      Cell Surface Display Techniques*; Fungal Proteins/*metabolism ; Fungi/*metabolism ; Membrane Proteins/*metabolism; Biotechnology ; Cell Wall/chemistry ; Cell Wall/metabolism ; Fungal Proteins/genetics ; Fungi/genetics ; Glycosylphosphatidylinositols/genetics ; Glycosylphosphatidylinositols/metabolism ; Membrane Proteins/genetics ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism
    • نبذة مختصرة :
      Protein display approaches have been useful to endow the cell surface of yeasts with new catalytic activities so that they can act as enhanced whole-cell biocatalysts. Despite their biotechnological potential, protein display technologies remain poorly developed for filamentous fungi. The lignocellulolytic character of some of them coupled to the cell surface biosynthesis of valuable molecules by a single or a cascade of several displayed enzymes is an appealing prospect. Cell surface protein display consists in the co-translational fusion of a functional protein (passenger) to an anchor one, usually a cell-wall-resident protein. The abundance, spacing, and local environment of the displayed enzymes-determined by the relationship of the anchor protein with the structure and dynamics of the engineered cell wall-are factors that influence the performance of display-based biocatalysts. The development of protein display strategies in filamentous fungi could be based on the field advances in yeasts; however, the unique composition, structure, and biology of filamentous fungi cell walls require the customization of the approach to those microorganisms. In this prospective review, the cellular bases, the design principles, and the available tools to foster the development of cell surface protein display technologies in filamentous fungi are discussed.
    • Grant Information:
      245750 Consejo Nacional de Ciencia y Tecnología
    • Contributed Indexing:
      Keywords: Cell surface engineering; Cell wall; Filamentous fungi; GPI-CWP; PIR proteins; Protein display
    • الرقم المعرف:
      0 (Fungal Proteins)
      0 (Glycosylphosphatidylinositols)
      0 (Membrane Proteins)
      0 (Recombinant Fusion Proteins)
    • الموضوع:
      Date Created: 20190731 Date Completed: 20191206 Latest Revision: 20191217
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1007/s00253-019-10026-7
    • الرقم المعرف:
      31359105