Protein Expression Analysis in Reversible Photobleached Cells of Scenedesmus vacuolatus after High Temperature Stress.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Hot Temperature* ; Photobleaching* ; Stress, Physiological*; Heat-Shock Proteins/*genetics ; Scenedesmus/*physiology ; Scenedesmus/*radiation effects; Algal Proteins/genetics ; Chromatography, Liquid ; Gene Expression Regulation, Plant ; Proteome ; Proteomics/methods ; Scenedesmus/ultrastructure ; Tandem Mass Spectrometry

We have analyzed protein expression in the bleached small vegetative cells of synchronous Scenedesmus vacuolatus to investigate how unicellular algae lived through stress. These cells were subjected to heat treatment (46.5 °C for 1h in dark condition) and then cultured under continuous illumination for 24 h. Flow cytometry analysis of the chlorophyll autofluorescence intensity of S. vacuolatus cells indicated that heat-treated cells were completely bleached within 24 h of light cultivation. Transmission electron microscopy (TEM) images showed that bleached cells maintained thylakoid membrane structure, but with lower contrast. The bleached cells regained green color after 72 h, along with a recovery in contrast, which indicated a return of photosynthetic ability. Two-dimensional gel electrophoresis (2DE) showed that the protein expression patterns were very difference between control and bleached cells. ATP synthase subunits and glutamine synthetase were down-regulated among the many differences, while some of phototransduction, stress response proteins were up-regulated in bleached cells, elucidating bleached cells can undergo changes in their biochemical activity, and activate some stress response proteins to survive the heat stress and then revive. In addition, small heat shock proteins (HSPs), but not HSP40 and HSP70 family proteins, protected the bleaching cells.

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Keywords: ATP synthase; chlorophyll; heat shock protein (HSP); heat stress; photobleaching; protein identification

0 (Algal Proteins)
0 (Heat-Shock Proteins)
0 (Proteome)

Date Created: 20190627 Date Completed: 20191206 Latest Revision: 20231012

20250114

PMC6627643

10.3390/ijms20123082

31238532