What's It All Good For?

ENZYME inhibitors; IMP dehydrogenase; CHEMICAL inhibitors; CRYPTOSPORIDIUM parvum; PROTEIN binding; NAD (Coenzyme); NAD(P)H dehydrogenases; BINDING sites; BIOCHEMISTRY

The article reports on the study which designs small-molecule screen for inhibitors of the inosine-5'-monophosphate dehydrogenenase (IMPDH) to investigate the characteristics of Cryptosporidium parvum. The researchers screen the kinetically defined conditions of IMP site to examine the random order of substrate binding and rate-limiting hydrolysis of the covalent enzymes. It reveals that 4 out 10 enzyme candidates for paromomycin are potent inhibitors of Cryptosporidium parvum are collected at the nicotinamide adenine dinucleotide (NAD) site.