Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Identification and characterisation of a glycine-rich RNA-binding protein as an endogenous suppressor of RNA silencing from Nicotiana glutinosa.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin, New York, Springer-Verlag [etc.]
    • الموضوع:
      RNA Interference*; Plant Diseases/*virology ; RNA, Small Interfering/*genetics ; RNA-Binding Proteins/*metabolism ; Nicotiana/*genetics; Agrobacterium ; Amino Acid Sequence ; Arginine ; Cucumovirus/physiology ; Genes, Reporter ; Phylogeny ; Plant Leaves/genetics ; Plant Leaves/virology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; RNA-Binding Proteins/genetics ; Sequence Alignment ; Nicotiana/virology ; Tobacco Mosaic Virus/physiology
    • نبذة مختصرة :
      Main Conclusion: This study shows that NgRBP suppresses both local and systemic RNA silencing induced by sense- or double-stranded RNA, and the RNA binding activity is essential for its function. To counteract host defence, many plant viruses encode viral suppressors of RNA silencing targeting various stages of RNA silencing. There is increasing evidence that the plants also encode endogenous suppressors of RNA silencing (ESR) to regulate this pathway. In this study, using Agrobacterium infiltration assays, we characterized NgRBP, a glycine-rich RNA-binding protein from Nicotiana glutinosa, as an ESR. Our results indicated that NgRBP suppressed both local and systemic RNA silencing induced by sense- or double-stranded RNA. We also demonstrated that NgRBP could promote Potato Virus X (PVX) infection in N. benthamiana. NgRBP knockdown by virus-induced gene silencing enhanced PVX and Cucumber mosaic virus resistance in N. glutinosa. RNA immunoprecipitation and electrophoretic mobility shift assays showed that NgRBP bound to GFP mRNA, dsRNA rather than siRNA. These findings provide the evidence that NgRBP acts as an ESR and the RNA affinity of NgRBP plays the key role in its ESR activity. NgRBP responds to multiple signals such as ABA, MeJA, SA, and Tobacco mosaic virus infection. Therefore, it could participate in the regulation of gene expression under specific conditions.
    • Grant Information:
      ZR2015CM018 Natural Science Foundation of Shandong Province; ZR2018LC009 Natural Science Foundation of Shandong Province; 2017ws426 Shandong Medical and Health Science and Technology Development Plan Project; 2016ZX08001-002 Genetically Modified Organism Breeding Major Projects of China
    • Contributed Indexing:
      Keywords: Glycine-rich RNA-binding protein; NgRBP; RNA silencing suppressor
    • الرقم المعرف:
      0 (Plant Proteins)
      0 (RNA, Small Interfering)
      0 (RNA-Binding Proteins)
      144517-63-5 (GRP protein, Nicotiana sylvestris)
      94ZLA3W45F (Arginine)
    • الموضوع:
      Date Created: 20190307 Date Completed: 20190520 Latest Revision: 20231213
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1007/s00425-019-03122-5
    • الرقم المعرف:
      30840177