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Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Calnexin/*metabolism ; Diacylglycerol O-Acyltransferase/*metabolism ; Endoplasmic Reticulum/*metabolism; Adipocytes/cytology ; Adipocytes/metabolism ; Animals ; COS Cells ; Cells, Cultured ; Chlorocebus aethiops ; HEK293 Cells ; Humans ; Mice ; Triglycerides/metabolism
    • نبذة مختصرة :
      Triacylglycerol synthesis is catalyzed by acyl CoA:diacylglycerol acyltransferase-2 (DGAT2). DGAT2 is an integral membrane protein that is localized to the endoplasmic reticulum and interacts with lipid droplets. Using BioId, a method to detect proximal and interacting proteins, we identified calnexin as a DGAT2-interacting protein. Co-immunoprecipitation and proximity ligation assays confirmed this finding. We found that calnexin-deficient mouse embryonic fibroblasts had reduced intracellular triacylglycerol levels and fewer large lipid droplets (>1.0 μm2 area). Despite the alterations in triacylglycerol metabolism, in vitro DGAT2 activity, localization and protein stability were not affected by the absence of calnexin.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Grant Information:
      FRN# 123385 Canada CIHR
    • الرقم المعرف:
      0 (Triglycerides)
      139873-08-8 (Calnexin)
      EC 2.3.1.20 (DGAT2 protein, mouse)
      EC 2.3.1.20 (Diacylglycerol O-Acyltransferase)
    • الموضوع:
      Date Created: 20190108 Date Completed: 20191018 Latest Revision: 20200309
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC6322727
    • الرقم المعرف:
      10.1371/journal.pone.0210396
    • الرقم المعرف:
      30615684