Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum.

Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag [etc.]

Phosphoric Monoester Hydrolases/*metabolism ; Plant Proteins/*metabolism ; Plants, Genetically Modified/*metabolism ; Nicotiana/*metabolism ; Transcription Factors/*metabolism; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Gene Expression Regulation, Plant ; Phosphoric Monoester Hydrolases/genetics ; Plant Proteins/genetics ; Plants, Genetically Modified/genetics ; Nicotiana/genetics ; Transcription Factors/genetics

Main Conclusion: Transgenic tobacco plants overexpressing the monoterpene alcohol geraniol synthase exhibit hypersensitivity to thermal stress, possibly due to suppressed sugar metabolism and transcriptional regulation of genes involved in thermal stress tolerance. Monoterpene alcohols function in plant survival strategies, but they may cause self-toxicity to plants due to their hydrophobic and highly reactive properties. To explore the role of these compounds in plant stress responses, we assessed transgenic tobacco plants overexpressing the monoterpene alcohol geraniol synthase (GES plants). Growth, morphology and photosynthetic efficiency of GES plants were not significantly different from those of control plants (wild-type and GUS-transformed plants). While GES plants' direct defenses against herbivores or pathogens were similar to those of control plants, their indirect defense (i.e., attracting herbivore enemy Nesidiocoris tenuis) was stronger compared to that of control plants. However, GES plants were susceptible to cold stress and even more susceptible to extreme heat stress (50 °C), as shown by decreased levels of sugar metabolites, invertase activity and its products (Glc and Fru), and leaf starch granules. Moreover, GES plants showed decreased transcription levels of the WRKY33 transcription factor gene and an aquaporin gene (PIP2). The results of this study show that GES plants exhibit enhanced indirect defense ability against herbivores, but conversely, GES plants exhibit hypersensitivity to heat stress due to suppressed sugar metabolism and gene regulation for thermal stress tolerance.

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16K07407 Japan Society for the Promotion of Science; 18H04630 Grant-in-Aid for Scientific Research on Innovative Areas; 18H04786 Grant-in-Aid for Scientific Research on Innovative Areas

Keywords: Geraniol; Monoterpene; Sugar metabolites; Thermal stress; Tobacco

0 (Arabidopsis Proteins)
0 (Plant Proteins)
0 (Transcription Factors)
0 (WRKY33 protein, Arabidopsis)
EC 3.1.3.2 (Phosphoric Monoester Hydrolases)

Date Created: 20181128 Date Completed: 20190226 Latest Revision: 20231213

20231215

10.1007/s00425-018-3054-z

30478473