Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Fc-gamma receptor expression profile in a North-Indian cohort of pediatric-onset systemic lupus erythematosus: An observational study.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Wiley on behalf of the Asia Pacific League of Associations for Rheumatology Country of Publication: England NLM ID: 101474930 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1756-185X (Electronic) Linking ISSN: 17561841 NLM ISO Abbreviation: Int J Rheum Dis
    • بيانات النشر:
      Original Publication: [Oxford, UK] : Wiley on behalf of the Asia Pacific League of Associations for Rheumatology
    • الموضوع:
      Lupus Erythematosus, Systemic/*immunology ; Receptors, IgG/*immunology; Adolescent ; Age of Onset ; Biomarkers/blood ; Case-Control Studies ; Child ; Child, Preschool ; Cross-Sectional Studies ; Female ; Flow Cytometry ; GPI-Linked Proteins/blood ; GPI-Linked Proteins/immunology ; Humans ; India/epidemiology ; Infant ; Infant, Newborn ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/diagnosis ; Lupus Erythematosus, Systemic/epidemiology ; Male ; Monocytes/immunology ; Neutrophils/immunology ; Receptors, IgG/blood
    • نبذة مختصرة :
      Background: Polymorphisms in the Fcγ-receptor (FcγR) have been associated with increased susceptibility to systemic lupus erythematosus (SLE). There is a paucity of data on FcγR expression pattern in pediatric subjects with SLE. The aim of the study was to assess the expression of various FcγRs by flow cytometry in children with pediatric-onset SLE (pSLE).
      Methods: Thirty-one children aged 0-15 years fulfilling 2012 Systemic Lupus International Collaborating Clinics Classification Criteria for SLE were enrolled. Disease-active (n = 14) and the inactive group were delineated using the SLE Disease Activity Index (SLEDAI). Thirteen age- and sex-matched controls were also enrolled. Blood samples of cases and controls were assessed for CD64, CD32B and CD16 expression on B lymphocytes, neutrophils and monocytes by flow cytometry using standard techniques. Median fluorescence intensity (MFI) and percentage expression were calculated using the FACS DIVA software and Kaluza software.
      Results: Median fluorescence intensity and percentage expression of CD64 on monocytes (MFI: 1.71 vs 1.51, P = 0.86) and neutrophils (MFI: 0.42 vs 0.64, P = 0.3) were comparable between patients and controls. MFIs of CD16 expression on neutrophils (3.47 vs 11.4, P = 0.05) and monocytes (1.28 vs 3.45, P = 0.07) were lower in patients compared to controls. CD32B expression on lymphocytes (MFI: 0.56 vs 1.37; % expression:18.3% vs 12.32%) was also comparable between cases and controls. Expression of CD64, CD16, and CD32B were also comparable between patients with active and inactive disease.
      Conclusion: No significant differences were observed in FcγR expression between patients and controls. However, the overall trends of FcγR expression and decreased CD16 on monocytes and neutrophils are in consonance with data from larger cohorts of adult SLE patients.
      (© 2018 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd.)
    • Grant Information:
      Special research grant for MD/MS/DM/MCh thesis from PGIMER, Chandigarh, India
    • Contributed Indexing:
      Keywords: CD16; CD32B; CD64; Fc-gamma receptor; North India; flow cytometry; pediatric-onset systemic lupus erythematosus
    • الرقم المعرف:
      0 (Biomarkers)
      0 (FCGR3B protein, human)
      0 (Fc gamma receptor IIB)
      0 (GPI-Linked Proteins)
      0 (Receptors, IgG)
    • الموضوع:
      Date Created: 20181107 Date Completed: 20190729 Latest Revision: 20190729
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1111/1756-185X.13403
    • الرقم المعرف:
      30399647