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Prevalence of different carbapenemase genes among carbapenem-resistant Acinetobacter baumannii blood isolates in Taiwan.

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  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101585411 Publication Model: eCollection Cited Medium: Internet ISSN: 2047-2994 (Electronic) Linking ISSN: 20472994 NLM ISO Abbreviation: Antimicrob Resist Infect Control Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central
    • الموضوع:
      Bacteremia* ; beta-Lactam Resistance*; Acinetobacter Infections/*epidemiology ; Acinetobacter Infections/*microbiology ; Acinetobacter baumannii/*drug effects ; Acinetobacter baumannii/*genetics ; Bacterial Proteins/*genetics ; Carbapenems/*pharmacology ; beta-Lactamases/*genetics; Acinetobacter baumannii/isolation & purification ; Bacterial Proteins/biosynthesis ; Carbapenems/therapeutic use ; Electrophoresis, Gel, Pulsed-Field ; Humans ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Prevalence ; Public Health Surveillance ; Taiwan/epidemiology ; beta-Lactamases/biosynthesis
    • نبذة مختصرة :
      Background: Although the prevalence of the carbapenem-resistant A. baumannii (CRAB) has increased in Taiwan, few studies have elucidated the prevalence of different carbapenemase genes in Taiwan. The first objective of this study was to identify the types and prevalence of different carbapenemase genes, and the second objective was to determine the carbapenem antimicrobial susceptibility of carbapenemase producing isolates.
      Methods: In total, 269 CRAB blood isolates from four medical centres in Taiwan from 1/1/2009 to 31/12/2013 were analysed. Antimicrobial susceptibilities were determined using the Vitek 2 system. Carbapenemase genes were identified by polymerase chain reaction (PCR) and sequencing. Pulsed-field gel electrophoresis (PFGE) was used to identify the different pulsotypes.
      Results: All 269 CRAB isolates had the bla OXA-51 -like gene, while 237 (88.1%) had the bla OXA-23 -like gene, and 11 (4.09%) had the bla OXA-24 -like gene. Twenty-one CRAB isolates (7.81%) contained only the bla OXA-51 -like gene. None of the isolates had the bla OXA-58 -like gene or the metallo-β-lactamases (MBL)-encoding genes. In 28.69% of isolates with the bla OXA-23 -like gene and 90.91% of isolates with the bla OXA-24 -like gene, the minimum inhibitory concentrations (MICs) for imipenem were 64 mg/L or more. In 37.55% of isolates with the bla OXA-23 -like gene and 100% of isolates with the bla OXA-24 -like gene, meropenem MICs were 64 mg/L or more. PFGE analyses indicated that six highly similar genomes which harbored the bla OXA-24 -like gene came from three different medical centres.
      Conclusion: Our study determined the prevalence of CRAB, the types and prevalence of carbapenemase genes, carbapenem susceptibility among CRAB isolates, and documented that the bla OXA-24 -like gene had greater resistance to carbapenem than the bla OXA-23 -like gene. We also demonstrated inter-hospital transmission of the highly resistant bla OXA-24 -like gene.
      Competing Interests: This study was approved by Institutional Review Board, MacKay Memorial Hospital, Taipei, Taiwan. Protocol no. 14MMHIS125.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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    • Contributed Indexing:
      Keywords: Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex; Carbapenem-resistant Acinetobacter baumannii (CRAB); blaOXA-23-like; blaOXA-24-like; blaOXA-51-like
    • الرقم المعرف:
      0 (Bacterial Proteins)
      0 (Carbapenems)
      EC 3.5.2.6 (beta-Lactamases)
      EC 3.5.2.6 (carbapenemase)
    • الموضوع:
      Date Created: 20181020 Date Completed: 20190918 Latest Revision: 20190918
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC6182870
    • الرقم المعرف:
      10.1186/s13756-018-0410-5
    • الرقم المعرف:
      30338061