Characterization of Der f 22 - a paralogue of the major allergen Der f 2.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Allergens/*metabolism ; Antigens, Dermatophagoides/*metabolism ; Arthropod Proteins/*metabolism; Allergens/chemistry ; Allergens/classification ; Amino Acid Sequence ; Antigens, Dermatophagoides/chemistry ; Antigens, Dermatophagoides/classification ; Arthropod Proteins/chemistry ; Arthropod Proteins/classification ; Base Sequence ; Blotting, Southern ; Enzyme-Linked Immunosorbent Assay ; Immunoglobulin E/chemistry ; Immunoglobulin E/metabolism ; Molecular Sequence Data ; Phylogeny ; Proteomics/methods

We previously identified an expressed sequence tag clone, Der f 22, showing 41% amino acid identity to published Der f 2, and show that both genes are possible paralogues. The objective of this study was to characterize the genomic, proteomic and immunological functions Der f 22 and Der f 2. The full-length sequence of Der f 2 and Der f 22 coded for mature proteins of 129 and 135 amino acids respectively, both containing 6 cysteine residues. Phylogenetic analysis of known group 2 allergens and their homologues from our expressed sequence tag library showed that Der f 22 is a paralogue of Der f 2. Both Der f 2 and Der f 22 were single gene products with one intron. Both allergens showed specific IgE-binding to over 40% of the atopic patients, with limited of cross-reactivity. Both allergens were detected at the gut region of D. farinae by immunostaining. Der f 22 is an important allergen with significant IgE reactivity among the atopic population, and should be considered in the diagnostic panel and evaluated as future hypoallergen vaccine therapeutic target.

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Research Scholarship International National University of Singapore (NUS); NMRC/1150/2008 International MOH | National Medical Research Council (NMRC)

0 (Allergens)
0 (Antigens, Dermatophagoides)
0 (Arthropod Proteins)
0 (Dermatophagoides farinae antigen f 2)
37341-29-0 (Immunoglobulin E)

Date Created: 20180808 Date Completed: 20191018 Latest Revision: 20191018

20231215

PMC6079044

10.1038/s41598-018-30224-z

30082894