Two Drosophila Neuropeptide Y-like Neurons Define a Reward Module for Transforming Appetitive Odor Representations to Motivation.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Appetite/*genetics ; Drosophila Proteins/*genetics ; Drosophila melanogaster/*genetics ; Feeding Behavior/*physiology ; Neuropeptides/*genetics; Animals ; Appetite/physiology ; Dopamine/genetics ; Dopamine/metabolism ; Dopaminergic Neurons/metabolism ; Drosophila melanogaster/physiology ; Larva/genetics ; Larva/physiology ; Motivation/genetics ; Neuropeptide Y/genetics ; Odorants ; Rhombencephalon/metabolism ; Rhombencephalon/physiology ; Smell/genetics ; Smell/physiology

Neuropeptides, many of which are conserved among vertebrate and invertebrate animals, are implicated in the regulation of motivational states that selectively facilitate goal-directed behaviors. After a brief presentation of appetitive odors, Drosophila larvae display an impulsive-like feeding activity in readily accessible palatable food. This innate appetitive response may require coordinated signaling activities of dopamine (DA) and neuropeptide F (NPF; a fly homolog of neuropeptide Y). Here we provide anatomical and functional evidence, at single-cell resolution, that two NPF neurons define a reward module in the highest-order brain region for cognitive processing of food-related olfactory representations. First, laser lesioning of these NPF neurons abolished odor induction of appetitive arousal, while their genetic activation mimicked the behavioral effect of appetitive odors. Further, a circuit analysis shows that each of the two NPF neurons relays its signals to a subset of target neurons in the larval hindbrain-like region. Finally, the NPF neurons discriminatively responded to appetitive odor stimuli, and their odor responses were blocked by targeted lesioning of a pair of dopaminergic third-order olfactory neurons that appear to be presynaptic to the NPF neurons. Therefore, the two NPF neurons contribute to appetitive odor induction of impulsive-like feeding by selectively decoding DA-encoded ascending olfactory inputs and relaying NPF-encoded descending motivational outputs for behavioral execution.

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R01 DC013333 United States DC NIDCD NIH HHS; R21 DK102209 United States DK NIDDK NIH HHS

0 (Drosophila Proteins)
0 (Neuropeptide Y)
0 (Neuropeptides)
0 (neuropeptide F, Drosophila)
VTD58H1Z2X (Dopamine)

Date Created: 20180805 Date Completed: 20191016 Latest Revision: 20191016

20240628

PMC6076267

10.1038/s41598-018-30113-5

30076343