Cellular adaptive response and regulation of HIF after low dose gamma-radiation exposure.

Publisher: Taylor & Francis Country of Publication: England NLM ID: 8809243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-3095 (Electronic) Linking ISSN: 09553002 NLM ISO Abbreviation: Int J Radiat Biol Subsets: MEDLINE

Original Publication: London ; New York : Taylor & Francis, [c1988-

Basic Helix-Loop-Helix Transcription Factors/*metabolism ; Gamma Rays/*adverse effects; Apoptosis/radiation effects ; Cell Survival/radiation effects ; DNA Damage ; Dose-Response Relationship, Radiation ; Humans ; MCF-7 Cells

Purpose: Cellular damage due to low dose of γ-radiation (≤0.1 Gy) is generally extrapolated from observing the effects at higher doses. These estimations are not accurate. This has led to uncertainties while assessing the radiation risk factors at low doses. Although there are reports on the radiation induced adaptive response, the mechanism of action is not fully elucidated, leading to the uncertainties. One of the outcomes of low dose radiation exposure is believed to be an adaptive response. The mechanism of adaptive response is not fully understood. Therefore, the study was undertaken to understand the role of hypoxia inducible factor (HIF) on radiation induced adaptive response.
Materials and Methods: Human breast cancer cell line MCF-7 cells pre-exposed to low dose γ-radiation (0.1 Gy; priming dose) were exposed to 2 Gy (challenging dose) 8 h after the priming dose and studied for the adaptive response. Cell death was measured by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay and apoptosis was measured by fluorescence-activated cell sorting analysis. DNA damage was measured by alkaline comet assay. HIF transcription activity was assayed using transiently transfected plasmid having HIF consensus sequence and luciferase as the reporter gene.
Results: Cells when exposed to 0.1 Gy priming dose 8 h prior to the higher dose (2 Gy; challenging dose) results in lower amount of radiation induced damages compared to the cells exposed to 2 Gy alone. Cobalt chloride treatment in place of priming dose also results in the protection to cells when exposed to challenging dose. There was up-regulation of HIF activity when cells were exposed to priming dose, indicating the role of HIF in radiation induced response.
Conclusion: Results indicate the γ-radiation induced adaptive response. One of the mechanism proposed is up-regulation of HIF after low dose exposure, which protects the cells from damages when they are exposed to challenging dose of 2 Gy radiation.

Keywords: HIF; LNT; MTT assay; adaptive response; comet assay; low dose

0 (Basic Helix-Loop-Helix Transcription Factors)

Date Created: 20180627 Date Completed: 20190415 Latest Revision: 20190415

20221213

10.1080/09553002.2018.1493241

29944059