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The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966980 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2172 (Electronic) Linking ISSN: 14712172 NLM ISO Abbreviation: BMC Immunol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, [2000-
    • الموضوع:
      Colitis, Ulcerative/*genetics ; Interleukin-11/*genetics ; Intestinal Mucosa/*metabolism ; Proto-Oncogene Proteins c-fos/*genetics; Adult ; Colitis, Ulcerative/metabolism ; Colon/metabolism ; Colon/pathology ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene Expression ; Humans ; Interleukin-11/metabolism ; Intestinal Mucosa/pathology ; Iran ; Male ; Middle Aged ; Proto-Oncogene Proteins c-fos/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Young Adult
    • نبذة مختصرة :
      Background: Fra-1 (fosl1) belongs to the activator protein1 (AP-1) family inducing IL-11 expression in oxidative stress condition. IL-11 plays a pivotal role in protecting epithelial barriers integrity. In this study, we investigated the Fra-1 gene expression in the inflamed mucosa of patients with ulcerative colitis (UC) as well as its relation to IL-11 expression.
      Materials and Methods: We enrolled 20 patients and 20 healthy controls with definite UC based on the clinical criteria. Fra-1 gene expression in inflamed and non-inflamed colonic biopsies was determined by real-time polymerase chain reaction (RT-PCR). The IL-11 protein concentration was measured by Enzyme-Linked Immunosorbent Assay (ELISA) method. Pearson correlation was applied to calculate the relation between Fra-1 and IL-11.
      Results: An increased level of Fra-1 gene expression was observed in patients with mild ulcerative colitis. The protein concentration of IL-11 was also increased in mild UC patients. Conversely, a significant decrease of IL-11 protein level was detected in severe UC patients compared to control group.
      Conclusion: Oxidative stress in inflamed intestinal biopsies can induce fra-1 gene expression. Our findings suggest that Fra-1 transcription factor leads to the production of IL-11 protein in UC patients.
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    • Contributed Indexing:
      Keywords: Fra-1 gene; IL-11; Oxidative stress; Ulcerative colitis (UC)
    • الرقم المعرف:
      0 (IL11 protein, human)
      0 (Interleukin-11)
      0 (Proto-Oncogene Proteins c-fos)
      0 (fos-related antigen 1)
    • الموضوع:
      Date Created: 20180620 Date Completed: 20190708 Latest Revision: 20240327
    • الموضوع:
      20240327
    • الرقم المعرف:
      PMC6006762
    • الرقم المعرف:
      10.1186/s12865-018-0257-9
    • الرقم المعرف:
      29914371