Genomic characterization of GII.3 noroviruses isolated from children in Zhengzhou city, China, 2015/16.

Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE

Original Publication: Wien, New York, Springer-Verlag.

Caliciviridae Infections/*epidemiology ; Capsid Proteins/*genetics ; Gastroenteritis/*virology ; Norovirus/*genetics ; Norovirus/*isolation & purification ; RNA, Viral/*genetics; Base Sequence ; Child ; Child, Preschool ; China/epidemiology ; Cluster Analysis ; Feces/virology ; Genetic Variation/genetics ; Genotype ; Humans ; Norovirus/classification ; Phylogeny ; Sequence Alignment ; Sequence Analysis, RNA

In this study, we isolated, amplified and sequenced GII.3 norovirus (NoV) strains from children admitted to a department of pediatric gastroenterology presenting with acute gastroenteritis from September 2015 to March 2016. A total of 35 stool samples were collected and eight were GII.3 NoV positive, based on sequencing. The complete genome sequences were determined for two strains while partial genome sequences, encompassing approximately 3.2 kb of the 3´end, were generated for the six other strains. Genotyping analysis of all strains indicated that they belonged to GII P12/GII.3. Phylogenetic analysis indicated that these isolated strains could be divided into two clusters. Strains in cluster IV were the most frequently isolated and exhibited less intra-cluster variation in nucleotide sequences. Our study demonstrated that the GII.P12/GII.3 recombinant strain was the dominant GII.3 NoVs in Zhengzhou city.

0 (Capsid Proteins)
0 (RNA, Viral)

Date Created: 20180618 Date Completed: 20190111 Latest Revision: 20220409

20231215

10.1007/s00705-018-3905-8

29909464