Alterations of the levels of primary antioxidant enzymes in different grades of human astrocytoma tissues.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9423872 Publication Model: Print Cited Medium: Internet ISSN: 1029-2470 (Electronic) Linking ISSN: 10292470 NLM ISO Abbreviation: Free Radic Res Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: Yverdon, Switzerland : New York, NY : Harwood Academic ; distributed by STBS Ltd., c1994-

Antioxidants/*metabolism ; Astrocytoma/*metabolism ; Immunohistochemistry/*methods ; Neoplasm Grading/*methods ; Superoxide Dismutase/*metabolism; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Malignant astrocytoma is the most commonly occurring brain tumour in humans. Oxidative stress is implicated in the development of cancers. Superoxide dismutase 2 (SOD2) was found to exert tumour suppressive effect in basic research, but increased SOD2 protein level was associated with higher aggressiveness of human astrocytomas. However, studies reporting alterations of antioxidant enzymes in human astrocytomas often employed less accurate methods or included different types of tumours. Here we analysed the mRNA levels, activities, and protein levels of primary antioxidant enzymes in control brain tissues and various grades of astrocytomas obtained from 40 patients. SOD1 expression, SOD1 activity, and SOD1 protein level were lower in Grade IV astrocytomas. SOD2 expression was lower in low-grade (Grades I and II) and Grade III astrocytomas than in controls, but SOD2 expression and SOD2 protein level were higher in Grade IV astrocytomas than in Grade III astrocytomas. Although there was no change in SOD2 activity and a lower activity of citrate synthase (CS), the MnSOD:CS ratio increased in Grade IV astrocytomas compared with controls and low-grade astrocytomas. Furthermore, SOD1 activity, CS activity, SOD1 expression, GPX4 expression, and GPX4 protein level were inversely correlated with the malignancy, whereas catalase activity, catalase protein, SOD2 protein level, and the SOD2:CS ratio were positively correlated with the degree of malignancy. Lower SOD2:CS ratio was associated with poor outcomes for Grade IV astrocytomas. This is the first study to quantify changes of various primary antioxidant enzymes in different grades of astrocytomas at different levels concurrently in human astrocytomas.

Keywords: Astrocytoma; catalase; glutathione peroxidases; malignancy; superoxide dismutases

0 (Antioxidants)
EC 1.15.1.1 (Superoxide Dismutase)
EC 1.15.1.1 (superoxide dismutase 2)

Date Created: 20180605 Date Completed: 20190109 Latest Revision: 20190109

20231215

10.1080/10715762.2018.1483580

29862858