Physiological and Transcriptomic Analysis of a Chronologically Long-Lived Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering.

Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE

Publication: [Cham] : Springer
Original Publication: Totowa, NJ : Humana Press, c1994-

Metabolic Engineering* ; Transcriptome*; Saccharomyces cerevisiae/*genetics; Caloric Restriction ; Carbohydrates ; Evolution, Molecular ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; High-Throughput Screening Assays ; Mutagenesis ; Mutation ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae/metabolism

High-throughput aging studies with yeast as a model organism involve transposon-mutagenesis and yeast knockout collection, which have been pivotal strategies for understanding the complex cellular aging process. In this study, a chronologically long-lived Saccharomyces cerevisiae mutant was successfully obtained by using another high-throughput approach, evolutionary engineering, based on systematic selection in successive batch cultures under gradually increasing levels of caloric restriction. Detailed comparative physiological and transcriptomic analyses of the chronologically long-lived mutant and the reference strain revealed enhanced levels of respiratory metabolism, upregulation of genes related to carbohydrate metabolic processes, glycogen-trehalose pathways, stress response, and repression of protein synthesis-related genes in the long-lived mutant SRM11, already in the absence of caloric restriction. Interestingly, SRM11 had also significantly higher resistance to copper stress, and higher resistance to silver, ethanol, and 2-phenylethanol stresses than the reference strain. It also had lower ethanol production levels and an enhanced ethanol catabolism. To conclude, evolutionary engineering is another powerful high-throughput method for aging research, in addition to its widespread use in industrial strain development. Additionally, the interesting results revealed by this study about the potential relationship between longevity and various cellular properties are yet to be investigated further at molecular level.

FEBS Lett. 1996 Jul 29;390(3):258-60. (PMID: 8706872)
FASEB J. 2001 Mar;15(3):627-34. (PMID: 11259381)
Biochim Biophys Acta. 2012 Sep;1823(9):1580-93. (PMID: 22387373)
Plant Cell Physiol. 2000 Aug;41(8):960-7. (PMID: 11038056)
Community Dent Oral Epidemiol. 2009 Apr;37(2):97-103. (PMID: 18782331)
Mech Ageing Dev. 2008 Oct;129(10):580-92. (PMID: 18634819)
Environ Toxicol. 2008 Oct;23(5):599-606. (PMID: 18528910)
Aging Cell. 2014 Jun;13(3):457-67. (PMID: 24649827)
J Bacteriol. 1980 Sep;143(3):1384-94. (PMID: 6997270)
Appl Biochem Biotechnol. 2011 Aug;164(8):1278-91. (PMID: 21409410)
PLoS Genet. 2014 Feb 27;10(2):e1004168. (PMID: 24586198)
Mol Biosyst. 2011 Feb;7(2):394-402. (PMID: 21031176)
Microbiol Mol Biol Rev. 1998 Jun;62(2):334-61. (PMID: 9618445)
Science. 2005 Nov 18;310(5751):1193-6. (PMID: 16293764)
Cell Metab. 2012 Jul 3;16(1):55-67. (PMID: 22768839)
Free Radic Biol Med. 2008 Nov 15;45(10):1446-56. (PMID: 18804161)
Appl Environ Microbiol. 1989 Feb;55(2):468-77. (PMID: 2566299)
Mol Cells. 2017 Apr;40(4):307-313. (PMID: 28427248)
Nature. 2002 Jul 18;418(6895):344-8. (PMID: 12124627)
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9721-6. (PMID: 10449761)
Science. 2014 Oct 3;346(6205):71-5. (PMID: 25278607)
Biochim Biophys Acta. 2009 Oct;1790(10):1067-74. (PMID: 19539012)
Aging Cell. 2007 Feb;6(1):111-9. (PMID: 17266680)
Proc Natl Acad Sci U S A. 2004 Apr 13;101(15):5524-9. (PMID: 15044709)
Nat Genet. 1996 May;13(1):25-34. (PMID: 8673100)
Adv Biochem Eng Biotechnol. 2001;73:129-69. (PMID: 11816810)
Nature. 2003 Dec 11;426(6967):620. (PMID: 14668850)
FEMS Yeast Res. 2013 Dec;13(8):731-46. (PMID: 23992612)
Methods Enzymol. 1991;194:273-81. (PMID: 2005792)
Exp Gerontol. 2003 Jul;38(7):807-11. (PMID: 12855292)
J Vis Exp. 2009 May 06;(27):null. (PMID: 19421136)
Mol Cell Biol. 1994 Dec;14(12):8155-65. (PMID: 7969152)
Exp Gerontol. 2009 Sep;44(9):555-71. (PMID: 19539741)
J Biol Chem. 2013 Aug 16;288(33):23884-92. (PMID: 23846699)
Curr Opin Cell Biol. 2005 Apr;17(2):158-66. (PMID: 15780592)
Curr Biol. 2012 Sep 11;22(17):R741-52. (PMID: 22975005)
PLoS Genet. 2010 Jul 15;6(7):e1001024. (PMID: 20657825)
PLoS Genet. 2010 Apr 22;6(4):e1000921. (PMID: 20421943)
Eur J Ageing. 2013 May 15;10 (4):353-361. (PMID: 28804308)
Semin Cell Dev Biol. 2014 Dec;36:113-20. (PMID: 25148809)
FEMS Yeast Res. 2012 Mar;12(2):171-82. (PMID: 22136139)
Appl Microbiol Biotechnol. 2015 Aug;99(16):6775-89. (PMID: 26099330)
Genes Dev. 2006 Jan 15;20(2):174-84. (PMID: 16418483)
OMICS. 2010 Dec;14(6):629-38. (PMID: 20863251)
Nature. 2009 Jul 16;460(7253):392-5. (PMID: 19587680)
Mikrobiologiia. 2004 Jul-Aug;73(4):449-54. (PMID: 15521168)
Biotechnol Bioeng. 1996 Oct 5;52(1):109-21. (PMID: 18629857)
Evolution. 1995 Aug;49(4):649-659. (PMID: 28565142)
PLoS Genet. 2008 Jan;4(1):e13. (PMID: 18225956)
BMC Genomics. 2009 Dec 07;10:585. (PMID: 19968875)
Science. 2009 Oct 2;326(5949):140-4. (PMID: 19797661)
Mech Ageing Dev. 2008 Mar;129(3):138-53. (PMID: 18155270)
Aging Cell. 2007 Oct;6(5):649-62. (PMID: 17711561)
Mech Ageing Dev. 2007 Feb;128(2):187-95. (PMID: 17129597)
Cell Metab. 2012 Jul 3;16(1):18-31. (PMID: 22768836)
Cell Metab. 2010 Jan;11(1):35-46. (PMID: 20074526)
Science. 2000 Sep 22;289(5487):2126-8. (PMID: 11000115)
Cell Cycle. 2009 Apr 15;8(8):1256-70. (PMID: 19305133)
Aging Cell. 2011 Apr;10(2):185-90. (PMID: 21176090)
Cell Cycle. 2011 Dec 15;10(24):4230-6. (PMID: 22107964)
Aging Cell. 2007 Feb;6(1):95-110. (PMID: 17266679)
Science. 2010 Apr 16;328(5976):321-6. (PMID: 20395504)
Health Econ. 1999 Sep;8(6):485-96. (PMID: 10544314)
Science. 2001 Apr 13;292(5515):288-90. (PMID: 11292860)
Cell Metab. 2010 Jun 9;11(6):453-65. (PMID: 20519118)
Aging Cell. 2003 Apr;2(2):73-81. (PMID: 12882320)
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3148-53. (PMID: 14973188)
Heredity (Edinb). 1985 Aug;55 ( Pt 1):9-18. (PMID: 3930429)
Exp Gerontol. 1987;22(3):199-220. (PMID: 3113991)
Enzyme Microb Technol. 2000 Jun 1;26(9-10):706-714. (PMID: 10862876)
Science. 1991 Jun 21;252(5013):1668-75. (PMID: 2047876)
Biochemistry. 1988 Nov 15;27(23):8509-15. (PMID: 3064814)
Cell Cycle. 2011 May 1;10(9):1385-96. (PMID: 21447998)
J Microbiol. 2016 Jul;54(7):487-91. (PMID: 27350614)
Microbiol Rev. 1987 Sep;51(3):351-64. (PMID: 3312986)
Curr Biol. 1998 Sep 24;8(19):1091-4. (PMID: 9768365)
Cell. 2005 Nov 18;123(4):655-67. (PMID: 16286010)

39557 Istanbul Teknik Üniversitesi

Keywords: Aging; Caloric restriction; Chronological lifespan; Evolutionary engineering; Inverse metabolic engineering; Longevity; Saccharomyces cerevisiae; Stress resistance

0 (Carbohydrates)

Date Created: 20180521 Date Completed: 20181003 Latest Revision: 20181114

20250114

10.1007/s12033-018-0087-2

29779127