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Raman spectroscopy quantification of eumelanin subunits in natural unaltered pigments.

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  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Munksgaard Country of Publication: England NLM ID: 101318927 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-148X (Electronic) Linking ISSN: 17551471 NLM ISO Abbreviation: Pigment Cell Melanoma Res Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Oxford : Blackwell Munksgaard,
    • الموضوع:
      Melanins/*metabolism ; Spectrum Analysis, Raman/*methods; Animals ; Birds ; Calibration ; Feathers/chemistry ; Indoles/analysis ; Melanins/chemistry ; Pigmentation ; Reproducibility of Results
    • نبذة مختصرة :
      Eumelanins are large polymers composed of 5,6-dihydroxyindole (DHI) and 5,6-dihydroxyindole-2-carboxylic acid (DHICA). Reactive oxygen species generation is higher during the synthesis of DHI than during the synthesis of DHICA; thus, the production of eumelanins with high relative DHI contents may compromise the viability of cells and organisms. Here, we show that dispersive Raman spectroscopy allows a noninvasive direct detection and quantification of DHI and DHICA in unaltered eumelanins that can be applied to the analysis of natural pigments in biological tissues. Using synthetic eumelanins purely composed of DHI or DHICA, we develop a model to determine the differential contribution of these subunits to the Raman spectra of mixed eumelanins, and exemplify the predictive capacity of the model with natural eumelanins deposited in black and gray feathers of fowl. We found that the wavenumber ranges of 550-1,200 and 1,650-2,300 cm -1 are significant to predict the DHI:DHICA ratio in unknown samples.
      (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
    • Grant Information:
      RYC-2012-10237 International Spanish Ministry of Economy, Industry and Competitiveness (MINECO); SEV-2015-0522 International Spanish Ministry of Economy, Industry and Competitiveness (MINECO); FIS2016-80455-R International Spanish Ministry of Economy, Industry and Competitiveness (MINECO); EU-H2020 654148 International H2020 Framework; 424/C/2014 International Fundació la Marató de TV3; International Fundació Cellex Barcelona; International Generalitat de Catalunya
    • Contributed Indexing:
      Keywords: DHI; DHICA; ROS; Raman spectroscopy; eumelanins; melanogenesis
    • الرقم المعرف:
      0 (Indoles)
      0 (Melanins)
      12627-86-0 (eumelanin)
      4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
      Z3OC8499KG (5,6-dihydroxyindole)
    • الموضوع:
      Date Created: 20180509 Date Completed: 20190408 Latest Revision: 20190408
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1111/pcmr.12707
    • الرقم المعرف:
      29738111