Molecular mechanisms of an antimicrobial peptide piscidin (Lc-pis) in a parasitic protozoan, Cryptocaryon irritans.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Antimicrobial Cationic Peptides/*pharmacology ; Ciliophora/*genetics ; Protozoan Proteins/*genetics; Animals ; Antimicrobial Cationic Peptides/chemical synthesis ; Ciliophora/drug effects ; Gene Expression Profiling/methods ; Gene Expression Regulation/drug effects ; Perciformes/parasitology ; Sequence Analysis, RNA/methods ; Vesicular Transport Proteins

Background: Cryptocaryon irritans is an obligate parasitic ciliate protozoan that can infect various commercially important mariculture fish species and cause high lethality and economic loss. Current methods of controlling this parasite with chemicals or antibiotics are widely considered to be environmentally harmful. Piscidins with broad spectrum antibacterial, antifungal and antiviral activities were found to have potent activity against C. irritans. Little, however, has been understood about the killing mechanisms of piscidins in parasites.
Results: In total, 57.12, 50.44, 55.86 and 47.87 million raw reads were generated from untreated theront and trophont, and piscidin (Lc-pis) treated theront and trophont libraries, respectively. After de novo assembly, 966,609 unigenes were generated with an average length of 420 bp: among these, 618,629 unigenes showed identity with sequences in one or more databases, with some showing to be significantly manipulated by Lc-pis treatment. The species classification showed that more than 25.8% unigenes from trophonts were homologous to the large yellow croaker (Larimichthys crocea) and less than 3.8% unigenes from theronts were matched. The homologous unigenes demonstrated that the tissue from host could exist in trophonts and might be transported to parasite via vesicular transports. Our analysis showed that regulatory transcripts were involved in vesicular trafficking. Among transcripts induced by Lc-pis, most genes up-regulated in treated and untreated theronts were involved in cell migration and apoptosis related pathways. Few transcripts were found to be down-regulated in treated and untreated trophonts related to cell structure and migration after treatment.
Conclusions: This is the first transcriptome analysis of C. irritans exposed to Lc-pis, which enhanced the genomic resources and provided novel insights into molecular mechanisms of ciliates treated by cationic antimicrobial peptide. Our comprehensive transcriptome analysis can facilitate the identification of potential drug targets and vaccines candidates for controlling this devastating fish pathogen.

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31372504 National Natural Science Foundation of China; 41476118 National Natural Science Foundation of China; 2016NZ0001-4 Major Projects of Science and Technology Department of Fujian Province; 2015I11010045 Fujian Provincial Department of Science and Technology (CN); 2015N2002-3 Fujian Provincial Department of Science and Technology

Keywords: Cryptocaryon irritans; Fish disease; Molecular mechanism; Piscidin; Transcriptome

0 (Antimicrobial Cationic Peptides)
0 (Protozoan Proteins)
0 (Vesicular Transport Proteins)

Date Created: 20180429 Date Completed: 20181211 Latest Revision: 20200225

20250114

PMC6389114

10.1186/s12864-018-4565-5

29703140