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Metabolic Engineering of Glycyrrhizin Pathway by Over-Expression of Beta-amyrin 11-Oxidase in Transgenic Roots of Glycyrrhiza glabra.

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  • معلومة اضافية
    • المصدر:
      Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE
    • بيانات النشر:
      Publication: [Cham] : Springer
      Original Publication: Totowa, NJ : Humana Press, c1994-
    • الموضوع:
      Metabolic Engineering*; Cytochrome P-450 Enzyme System/*genetics ; Glycyrrhiza/*enzymology ; Glycyrrhizic Acid/*metabolism; Agrobacterium/genetics ; Biosynthetic Pathways ; Cytochrome P-450 Enzyme System/metabolism ; Gene Transfer Techniques ; Glycyrrhiza/genetics ; Glycyrrhiza/metabolism ; Glycyrrhizic Acid/analysis ; Oleanolic Acid/analogs & derivatives ; Oleanolic Acid/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Roots/genetics ; Plant Roots/metabolism ; Plants, Genetically Modified/metabolism
    • نبذة مختصرة :
      Glycyrrhiza glabra is one of the most important and well-known medicinal plants which produces various triterpene saponins such as glycyrrhizin. Beta-amyrin 11-oxidase (CYP88D6) plays a key role in engineering pathway of glycyrrhizin production and converts an intermediated beta-amyrin compound to glycyrrhizin. In this study, pBI121 GUS-9 :CYP88D6 construct was transferred to G. glabra using Agrobacterium rhizogene ATCC 15834. The quantitation of transgene was measured in putative transgenic hairy roots using qRT-PCR. The amount of glycyrrhizin production was measured by HPLC in transgenic hairy root lines. Gene expression analysis demonstrated that CYP88D6 was over-expressed only in one of transgenic hairy root lines and was reduced in two others. Beta-amyrin 24-hydroxylase (CYP93E6) was significantly expressed in one of the control hairy root lines. The amount of glycyrrhizin metabolite in over-expressed line was more than or similar to that of control hairy root lines. According to the obtained results, it would be recommended that multi-genes of glycyrrhizin biosynthetic pathway be transferred simultaneously to the hairy root in order to increase glycyrrhizin content.
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    • Contributed Indexing:
      Keywords: Agrobacterium rhizogenes; Beta-amyrin 24-hydroxylase; Composite plant; Hairy root; Triterpene saponin
    • الرقم المعرف:
      0 (Plant Proteins)
      6FO62043WK (Glycyrrhizic Acid)
      6SMK8R7TGJ (Oleanolic Acid)
      9035-51-2 (Cytochrome P-450 Enzyme System)
      KM8353IPSO (beta-amyrin)
    • الموضوع:
      Date Created: 20180425 Date Completed: 20180921 Latest Revision: 20200325
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC7090481
    • الرقم المعرف:
      10.1007/s12033-018-0082-7
    • الرقم المعرف:
      29687371