Phospholipid-flipping activity of P4-ATPase drives membrane curvature.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE

Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-

Adenosine Triphosphatases/*metabolism ; Cell Membrane/*enzymology ; Lipid Bilayers/*metabolism ; Membrane Transport Proteins/*metabolism ; Phosphatidylcholines/*metabolism; Adenosine Triphosphatases/genetics ; Cell Membrane/genetics ; HeLa Cells ; Humans ; Membrane Transport Proteins/genetics ; Phosphatidylcholines/genetics

P4-ATPases are phospholipid flippases that translocate phospholipids from the exoplasmic/luminal to the cytoplasmic leaflet of biological membranes. All P4-ATPases in yeast and some in other organisms are required for membrane trafficking; therefore, changes in the transbilayer lipid composition induced by flippases are thought to be crucial for membrane deformation. However, it is poorly understood whether the phospholipid-flipping activity of P4-ATPases can promote membrane deformation. In this study, we assessed membrane deformation induced by flippase activity via monitoring the extent of membrane tubulation using a system that allows inducible recruitment of Bin/amphiphysin/Rvs (BAR) domains to the plasma membrane (PM). Enhanced phosphatidylcholine-flippase activity at the PM due to expression of ATP10A, a member of the P4-ATPase family, promoted membrane tubulation upon recruitment of BAR domains to the PM This is the important evidence that changes in the transbilayer lipid composition induced by P4-ATPases can deform biological membranes.
(© 2018 The Authors.)

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Keywords: BAR domain; curvature; flippase; lipid; plasma membrane

0 (Lipid Bilayers)
0 (Membrane Transport Proteins)
0 (Phosphatidylcholines)
EC 3.6.1.- (Adenosine Triphosphatases)
EC 7.6.2.1 (ATP10A protein, human)

Date Created: 20180331 Date Completed: 20190107 Latest Revision: 20190502

20250114

PMC5920243

10.15252/embj.201797705

29599178