The presence of duck Tembusu virus in Thailand since 2007: A retrospective study.

Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE

Original Publication: Berlin : Blackwell Verlag

Communicable Diseases, Emerging/*veterinary ; Ducks/*virology ; Flavivirus/*isolation & purification ; Flavivirus Infections/*veterinary ; Poultry Diseases/*epidemiology; Animals ; Base Sequence ; China ; Communicable Diseases, Emerging/epidemiology ; Communicable Diseases, Emerging/virology ; Flavivirus/genetics ; Flavivirus Infections/epidemiology ; Flavivirus Infections/virology ; Genome, Viral ; Phylogeny ; Poultry Diseases/virology ; Retrospective Studies ; Thailand/epidemiology

Duck Tembusu virus (DTMUV), a newly emerging virus in ducks, was first reported in China in 2010. However, an unknown severe contagious disease associated with severe neurological signs and egg production losses in ducks, resembling to DTMUV infection, was observed in Thailand since 2007. To determine the presence of DTMUV in 2007, the clinical samples from affected ducks collected in 2007 were tested for DTMUV using pathological and virological analyses. Gross and histopathological lesions of affected ducks were mostly restricted to the ovary, brain and spinal cord, and correlated with the presence of flavivirus antigen in the brain and spinal cord samples. Subsequently, DTMUV was identified by RT-PCR and nucleotide sequencing of the polyprotein gene. Phylogenetic analysis of the polyprotein gene sequence revealed that the 2007 Thai DTMUV was a unique virus, belonged within DTMUV cluster 1, but distinctively separated from the Malaysian DTMUV, which was the most closely related DTMUV. It is interesting to note that the 2007 Thai DTMUV was genetically different from the currently circulating Thai and Chinese DTMUVs, which belonged to cluster 2. Our findings indicated that the 2007 Thai DTMUV emerged earlier from a common ancestor with the recently reported DTMUVs; however, it was genetically distinctive to any of the currently circulating DTMUVs. In conclusion, our data demonstrated the presence of DTMUV in the Thai ducks since 2007, prior to the first report of DTMUV in China in 2010. This study indicates that DTMUV may have circulated in the region long before 2010 and highlights high genetic diversity of DTMUVs in Asia.
(© 2018 Blackwell Verlag GmbH.)

RTA6080012 Thailand Research Fund; TRG5780010 Thailand Research Fund; Thai government budget for 2017

Keywords: Thailand; duck Tembusu virus; ducks; retrospective study

GENBANK MF621927; KX097990; KX097989; KR061333; KF573582; JF895923; KF192951; JF312912; JQ595407; KC990543; KC990542; JQ920426; KJ489355; JQ920420; AB917090; JQ920425; KM275940; KM066945; KT239021; KR869106; KT824876; KP742476; KX686572; KX686570; KX686571; JX477685; JX477686

Tembusu virus

Date Created: 20180310 Date Completed: 20181113 Latest Revision: 20201209

20240829

10.1111/tbed.12859

29520997