Cell surface chondroitin sulphate proteoglycan 4 (CSPG4) binds to the basement membrane heparan sulphate proteoglycan, perlecan, and is involved in cell adhesion.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print Cited Medium: Internet ISSN: 1756-2651 (Electronic) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE

Publication: : Abingdon, UK : Oxford University Press
Original Publication: Tokyo : Japanese Biochemical Society

Cell Adhesion*; Chondroitin Sulfate Proteoglycans/*metabolism ; Heparan Sulfate Proteoglycans/*metabolism ; Membrane Proteins/*metabolism; Binding Sites ; Chondroitin Sulfate Proteoglycans/chemistry ; Heparan Sulfate Proteoglycans/chemistry ; Humans ; Membrane Proteins/chemistry ; Protein Binding ; Tumor Cells, Cultured

Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007, as well as shed into the conditioned medium. CSPG4 from the two melanoma cell lines differed in the amount of chondroitin sulphate (CS) decoration, as well as the way the protein core was fragmented. In contrast, the CSPG4 expressed by a colon carcinoma cell line, WiDr, was predominantly as a protein core on the cell surface lacking glycosaminoglycan (GAG) chains. This study demonstrated that CSPG4 immunopurified from the melanoma cell lines formed a complex with perlecan synthesized by the same cultured cells. Mechanistic studies showed that CSPG4 bound to perlecan via hydrophobic protein-protein interactions involving multiple sites on perlecan including the C-terminal region. Furthermore, this study revealed that CSPG4 interacted with perlecan to support cell adhesion and actin polymerization. Together these data suggest a novel mechanism by which CSPG4 expressing cells might attach to perlecan-rich matrices so as those found in connective tissues and basement membranes.

Cancer Res. 1997 Jun 1;57(11):2130-6. (PMID: 9187109)
J Biol Chem. 1996 Apr 26;271(17):10079-86. (PMID: 8626565)
Proteomics. 2001 Dec;1(12):1534-41. (PMID: 11747213)
J Biol Chem. 1999 Jun 11;274(24):16831-7. (PMID: 10358027)
Microvasc Res. 2002 Jan;63(1):129-34. (PMID: 11749079)
Mol Biol Cell. 2004 Aug;15(8):3580-90. (PMID: 15181153)
Oncogene. 2008 Sep 4;27(39):5182-94. (PMID: 18469852)
Virchows Arch. 2002 Aug;441(2):165-73. (PMID: 12189507)
Am J Pathol. 1994 Nov;145(5):1070-81. (PMID: 7977639)
DNA Cell Biol. 2014 Dec;33(12):854-62. (PMID: 25166220)
Semin Cell Dev Biol. 2001 Apr;12(2):89-98. (PMID: 11292374)
Adv Cancer Res. 2010;109:73-121. (PMID: 21070915)
Eur J Biochem. 1997 Nov 15;250(1):39-46. (PMID: 9431988)
Matrix Biol. 2014 Apr;35:112-22. (PMID: 24509440)
J Cell Sci. 2001 Jun;114(Pt 12):2315-25. (PMID: 11493670)
J Cell Physiol. 1988 Nov;137(2):321-8. (PMID: 2973466)
J Neurosci. 2002 Apr 1;22(7):2792-803. (PMID: 11923444)
Breast Cancer Res. 2012 Apr 24;14 (2):R67. (PMID: 22531600)
Eur J Biochem. 1999 Feb;259(3):917-25. (PMID: 10092882)
J Cell Biochem. 1996 Dec 15;63(4):463-77. (PMID: 8978462)
Biochem J. 1994 Sep 15;302 ( Pt 3):625-39. (PMID: 7945186)
Nat Cell Biol. 1999 Dec;1(8):507-13. (PMID: 10587647)
Invest Ophthalmol Vis Sci. 2005 Dec;46(12):4365-71. (PMID: 16303921)
Mol Biol Cell. 1993 Nov;4(11):1097-108. (PMID: 8305732)
Cancer Res. 1995 May 15;55(10):2177-85. (PMID: 7743521)
Microcirculation. 2005 Mar;12(2):151-60. (PMID: 15824037)
Cancer Res. 1994 Nov 15;54(22):5771-4. (PMID: 7954396)
J Biol Chem. 2013 Feb 1;288(5):3289-304. (PMID: 23235151)
J Biol Chem. 1997 Apr 18;272(16):10769-76. (PMID: 9099729)
J Oral Pathol Med. 2008 May;37(5):287-93. (PMID: 18205742)
Dev Biol. 2010 Aug 15;344(2):1035-46. (PMID: 20599895)
Biomaterials. 2006 Mar;27(8):1341-5. (PMID: 16183113)
Mol Biol Cell. 1999 Oct;10(10):3373-87. (PMID: 10512873)
FEBS J. 2010 Oct;277(19):3904-23. (PMID: 20840587)
J Cell Biol. 1992 Nov;119(4):945-59. (PMID: 1385448)
Neuro Oncol. 2011 Aug;13(8):830-45. (PMID: 21798846)
Angiogenesis. 2003;6(3):241-9. (PMID: 15041800)
J Cell Physiol. 1998 Nov;177(2):299-312. (PMID: 9766527)
J Cell Biol. 1990 Dec;111(6 Pt 2):3177-88. (PMID: 2269670)
Dev Dyn. 2001 Oct;222(2):218-27. (PMID: 11668599)
Angiogenesis. 2014 Jan;17(1):61-76. (PMID: 23925489)
Mol Cell Neurosci. 2005 May;29(1):82-96. (PMID: 15866049)
Pharmacol Ther. 1993 Feb-Mar;57(2-3):259-90. (PMID: 8361995)
J Cell Biochem. 2002;86(4):726-36. (PMID: 12210739)
Colloids Surf B Biointerfaces. 2016 Dec 1;148:130-138. (PMID: 27591944)
Matrix Biol. 1999 Apr;18(2):163-78. (PMID: 10372557)
Biochemistry. 2010 Jul 6;49(26):5524-32. (PMID: 20507176)
Mol Biol Cell. 1996 Mar;7(3):383-96. (PMID: 8868467)
J Biol Chem. 1996 Oct 18;271(42):26110-6. (PMID: 8824254)
Mol Cell Biol. 1997 Apr;17(4):1938-46. (PMID: 9121441)
Cell. 1994 Dec 16;79(6):1005-13. (PMID: 7528102)
J Biol Chem. 2001 Jun 1;276(22):18786-94. (PMID: 11278606)
Curr Mol Med. 2010 Jun;10(4):419-29. (PMID: 20455858)
PLoS Biol. 2014 Nov 11;12 (11):e1001993. (PMID: 25387269)
Angiogenesis. 2013 Apr;16(2):309-27. (PMID: 23124902)
J Histochem Cytochem. 2003 Nov;51(11):1393-410. (PMID: 14566013)
Matrix Biol. 2009 Jun;28(5):284-91. (PMID: 19422911)
Neuron. 2011 May 26;70(4):661-73. (PMID: 21609823)
Mol Biol Cell. 1995 Dec;6(12):1819-32. (PMID: 8590808)
Ann Oncol. 1997 Dec;8(12):1257-61. (PMID: 9496392)
J Natl Cancer Inst. 2010 Oct 6;102(19):1496-512. (PMID: 20852124)
Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57. (PMID: 22004131)
J Cell Mol Med. 2011 May;15(5):1013-31. (PMID: 21155971)
Arthritis Rheumatol. 2016 Apr;68(4):868-79. (PMID: 26636652)
J Neurocytol. 2002 Jul-Aug;31(6-7):423-35. (PMID: 14501214)
Pathol Oncol Res. 2000;6(1):10-7. (PMID: 10749582)
Nat Struct Biol. 2001 Jul;8(7):634-40. (PMID: 11427896)
Cell Death Differ. 2008 May;15(5):899-907. (PMID: 18292781)
J Clin Invest. 1998 Oct 15;102(8):1599-608. (PMID: 9788974)
Microsc Res Tech. 2008 May;71(5):339-48. (PMID: 18300285)

R01 CA095287 United States CA NCI NIH HHS

0 (CSPG4 protein, human)
0 (Chondroitin Sulfate Proteoglycans)
0 (Heparan Sulfate Proteoglycans)
0 (Membrane Proteins)
143972-95-6 (perlecan)

Date Created: 20180221 Date Completed: 20180515 Latest Revision: 20190501

20250114

PMC5905647

10.1093/jb/mvy008

29462330