The superoxide dismutase genes might be required for appropriate development of the ovule after fertilization in Xanthoceras sorbifolium.

Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer, 1981-

Genes, Plant*; Fertilization/*genetics ; Ovule/*enzymology ; Ovule/*genetics ; Sapindaceae/*enzymology ; Sapindaceae/*genetics ; Superoxide Dismutase/*genetics; Exons/genetics ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Introns/genetics ; Isoenzymes/metabolism ; Ovule/cytology ; Phylogeny ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Pollination/genetics ; Promoter Regions, Genetic ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sapindaceae/cytology ; Superoxide Dismutase/metabolism

Key Message: Superoxide dismutase genes were expressed differentially along with developmental stages of fertilized ovules in Xanthoceras sorbifolium, and the XsMSD gene silencing resulted in the arrest of fertilized ovule development. A very small percentage of mature fruits (ca. 5%) are produced relative to the number of bisexual flowers in Xanthoceras sorbifolium because seeds and fruits are aborted at early stages of development after pollination. Reactive oxygen species (ROS) in plants are implicated in an extensive range of biological processes, such as programmed cell death and senescence. Superoxide dismutase (SOD) activity might be required to regulate ROS homeostasis in the fertilized ovules of X. sorbifolium. The present study identified five SOD genes and one SOD copper chaperone gene in the tree. Their transcripts were differentially expressed along different stages of fertilized ovule development. These genes showed maximum expression in the ovules at 3 days after pollination (DAP), a time point in which free nuclear endosperm and nucleus tissues rapidly develop. The XsCSD1, XsFSD1 and XsMSD contained seven, eight, and five introns, respectively. Analysis of the 5'-flanking region of XsFSD1 and XsMSD revealed many cis-acting regulatory elements. Evaluation of XsMSD gene function based on virus-induced gene silencing (VIGS) indicated that the gene was closely related to early development of the fertilized ovules and fruits. This study suggested that SOD genes might be closely associated with the fate of ovule development (aborted or viable) after fertilization in X. sorbifolium.

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30972344 National Natural Science Foundation of China; 31370611 National Natural Science Foundation of China; 31570680 National Natural Science Foundation of China; 6172028 Natural Science Foundation of Beijing Municipality

Keywords: Expression; Fertilized ovule development; Superoxide dismutase gene family; Xanthoceras sorbifolium

0 (Isoenzymes)
0 (Plant Proteins)
0 (RNA, Messenger)
EC 1.15.1.1 (Superoxide Dismutase)

Date Created: 20180202 Date Completed: 20180917 Latest Revision: 20220317

20250114

10.1007/s00299-018-2263-z

29387898