Multiplex highly sensitive immunochromatographic assay based on the use of nonprocessed antisera.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE

Original Publication: Heidelberg : Springer-Verlag, 2002-

Atrazine/*analysis ; Chromatography, Affinity/*instrumentation ; Herbicides/*analysis ; Immune Sera/*chemistry ; Sulfonamides/*analysis ; Triazines/*analysis; Animals ; Chromatography, Affinity/methods ; Equidae ; Equipment Design ; Food Analysis/instrumentation ; Food Analysis/methods ; Fruit and Vegetable Juices/analysis ; Goats ; Gold/chemistry ; Limit of Detection ; Metal Nanoparticles/chemistry ; Rabbits ; Reagent Strips/analysis

The format of an immunochromatographic multiassay is first proposed with native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. This format allows (1) the exclusion of purification and conjugation stages for specific antibodies and (2) significant reduction of the concentration of specific antibodies in the system. The independent use of specific antibodies and a conjugated marker provided a low detection limit and high signal intensity. The proposed format was implemented for the simultaneous detection of two herbicides. The instrumental limits for the detection of atrazine and chlorsulfuron were 0.1 and 0.7 ng/mL, respectively, and the analysis time was 20 min. The suitability of the test system for monitoring these herbicides in nontreated apple and blackcurrant juices is shown. The assay technique is simple, sensitive, and easily transferrable to any other antigen. Graphical abstract The proposed format of the immunochromatographic multiassay is based on the use of native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. In this way purification and conjugation stages for specific antibodies are excluded, and the concentrations of specific antibodies and the conjugated marker can be varied independently to obtain a low detection limit.

14-16-00149 Russian Science Foundation

Keywords: Gold nanoparticles; Herbicides; Immunoassay; Indirect labeling; Lateral flow assay; Test strips

0 (Herbicides)
0 (Immune Sera)
0 (Reagent Strips)
0 (Sulfonamides)
0 (Triazines)
7440-57-5 (Gold)
O6S620ML45 (chlorsulfuron)
QJA9M5H4IM (Atrazine)

Date Created: 20180122 Date Completed: 20180502 Latest Revision: 20181202

20250114

10.1007/s00216-018-0853-9

29353430