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Lead induces DNA damage and alteration of ALAD and antioxidant genes mRNA expression in construction site workers.

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  • معلومة اضافية
    • المصدر:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101282564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2154-4700 (Electronic) Linking ISSN: 19338244 NLM ISO Abbreviation: Arch Environ Occup Health Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2009- : Philadelphia : Taylor & Francis
      Original Publication: Washington, D.C. : Heldref Publications, c2006-
    • الموضوع:
      Construction Industry* ; DNA Damage*; Lead Poisoning/*genetics ; Occupational Exposure/*analysis ; Porphobilinogen Synthase/*genetics; Adult ; Comet Assay ; Humans ; Male ; Pakistan ; Polymerase Chain Reaction ; Polymorphism, Genetic/drug effects ; RNA, Messenger ; Young Adult
    • نبذة مختصرة :
      Oxidative stress and DNA damage are considered as possible mechanisms involved in lead toxicity. To test this hypothesis, DNA damage and expression variations of aminolevulinic acid dehydratase (ALAD), superoxide dismutase 2 (SOD2), and 8-oxoguanine DNA glycosylase 2a (OGG1-2a) genes was studied in a cohort of 100 exposed workers and 100 controls with comet assay and real-time polymerse chain reaction (PCR). Results indicated that increased number of comets was observed in exposed workers versus controls ( p < 0.001). After qPCR analysis, significant down-regulation in ALAD ( p < 0.0001), SOD2 ( p < 0.0001), and OGG1-2a ( p < 0.0001) level was observed in exposed workers versus controls. Additionally, a positive spearmen correlation was observed between ALAD versus SOD2 ( r = 0.402**, p < 0.001), ALAD versus OGG1-2a ( r = 0.235*, p < 0.05), and SOD2 versus OGG1-2a ( r = 0.292*, p < 0.05). This study showed that lead exposure induces DNA damage, which is accompanied by an elevated intensity of oxidative stress and expression variation of lead-related gene.
    • Contributed Indexing:
      Keywords: ALAD; DNA damage; OGG1-2a; SOD2; comet assay; lead; occupational exposure
    • الرقم المعرف:
      0 (RNA, Messenger)
      EC 4.2.1.24 (Porphobilinogen Synthase)
    • الموضوع:
      Date Created: 20180117 Date Completed: 20200106 Latest Revision: 20200106
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1080/19338244.2018.1428523
    • الرقم المعرف:
      29336731