Impaired Skin Barrier Due to Sebaceous Gland Atrophy in the Latent Stage of Radiation-Induced Skin Injury: Application of Non-Invasive Diagnostic Methods.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Radiation Injuries/*pathology ; Sebaceous Glands/*pathology ; Skin/*pathology; Animals ; Cytokines/metabolism ; Keratinocytes/metabolism ; Lipid Metabolism ; Male ; Mice ; Radiation Injuries/metabolism ; Serine Proteases/metabolism ; Skin/metabolism ; Skin/radiation effects

Radiation-induced skin injury can take the form of serious cutaneous damage and have specific characteristics. Asymptomatic periods are classified as the latent stage. The skin barrier plays a critical role in the modulation of skin permeability and hydration and protects the body against a harsh external environment. However, an analysis on skin barrier dysfunction against radiation exposure in the latent stage has not been conducted. Thus, we investigated whether the skin barrier is impaired by irradiation in the latent stage and aimed to identify the molecules involved in skin barrier dysfunction. We analyzed skin barrier function and its components in SKH1 mice that received 20 and 40 Gy local irradiation. Increased transepidermal water loss and skin pH were observed in the latent stage of the irradiated skin. Skin barrier components, such as structural proteins and lipid synthesis enzymes in keratinocyte, increased in the irradiated group. Interestingly, we noted sebaceous gland atrophy and increased serine protease and inflammatory cytokines in the irradiated skin during the latent period. This finding indicates that the main factor of skin barrier dysfunction in the latent stage of radiation-induced skin injury is sebaceous gland deficiency, which could be an intervention target for skin barrier impairment.
Competing Interests: The authors declare no conflict of interest.

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Keywords: radiation; sebaceous gland; skin barrier; skin pH; transepidermal water loss

0 (Cytokines)
EC 3.4.- (Serine Proteases)

Date Created: 20180111 Date Completed: 20180727 Latest Revision: 20181113

20221213

PMC5796134

10.3390/ijms19010185

29316698