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Enrichment of gamma-aminobutyric acid in bean sprouts: Exploring biosynthesis of plant metabolite using common household reagents.

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  • معلومة اضافية
    • المصدر:
      Publisher: John Wiley & sons Country of Publication: United States NLM ID: 100970605 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-3429 (Electronic) Linking ISSN: 14708175 NLM ISO Abbreviation: Biochem Mol Biol Educ Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2002- : Hoboken, NJ : John Wiley & sons
      Original Publication: Oxford, UK : Elsevier, c2000-
    • الموضوع:
      Learning* ; Students*; Glutamic Acid/*metabolism ; Indicators and Reagents/*metabolism ; Vigna/*metabolism ; gamma-Aminobutyric Acid/*biosynthesis; Glutamic Acid/chemistry ; Indicators and Reagents/chemistry ; Universities
    • نبذة مختصرة :
      The enrichment of plant foods with gamma-aminobutyric acid (GABA) is currently an interesting issue in the field of nutraceuticals and can be used as an experiment for upper-division undergraduate students. Here, an interdisciplinary hands-on experiment to produce GABA-enriched mung bean sprouts using common household reagents is described. Based on the GABA biosynthesis pathways, two key factors, being the availability of l-glutamic acid and the acidification of the germination environment, were chosen for the study of the effects on the enhancement of GABA levels. The activities not only led students to a deeper understanding of biochemistry contents, but also gave the students the opportunity to work with experimental design, analytical chemistry, and statistical data analysis. Furthermore, since mung bean sprouts are familiar foods and the reagents used for germination are easily obtainable and generally recognized as safe, the optimal protocol investigated in the lab could be further applied to the production of bean sprouts with enhanced nutritional values in everyday life, promoting the transfer of knowledge learned in school to practical environments such as home and community. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(2):155-161, 2018.
      (© 2017 The International Union of Biochemistry and Molecular Biology.)
    • Contributed Indexing:
      Keywords: GABA; biosynthesis; mung bean sprouts
    • الرقم المعرف:
      0 (Indicators and Reagents)
      3KX376GY7L (Glutamic Acid)
      56-12-2 (gamma-Aminobutyric Acid)
    • الموضوع:
      Date Created: 20171208 Date Completed: 20190327 Latest Revision: 20190327
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1002/bmb.21100
    • الرقم المعرف:
      29215197