Central NPY-Y5 sub-receptor partially functions as a mediator of NPY-induced hypothermia and affords thermotolerance in heat-exposed fasted chicks.

Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE

Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-

Heat-Shock Response*; Brain/*metabolism ; Fasting/*metabolism ; Hypothermia/*metabolism ; Neuropeptide Y/*pharmacology; Adaptation, Physiological ; Animals ; Blood Glucose/metabolism ; Body Temperature ; Brain/drug effects ; Chickens ; Fasting/physiology ; Heat-Shock Proteins/metabolism ; Hypothermia/etiology ; Hypothermia/physiopathology ; Male ; Naphthalenes/pharmacology ; Neuropeptide Y/toxicity ; Pyrimidines/pharmacology ; Receptors, Neuropeptide Y/metabolism

Exposure of chicks to a high ambient temperature (HT) has previously been shown to increase neuropeptide Y (NPY) mRNA expression in the brain. Furthermore, it was found that NPY has anti-stress functions in heat-exposed fasted chicks. The aim of the study was to reveal the role of central administration of NPY on thermotolerance ability and the induction of heat-shock protein (HSP) and NPY sub-receptors (NPYSRs) in fasted chicks with the contribution of plasma metabolite changes. Six- or seven-day-old chicks were centrally injected with 0 or 375 pmol of NPY and exposed to either HT (35 ± 1°C) or control thermoneutral temperature (CT: 30 ± 1°C) for 60 min while fasted. NPY reduced body temperature under both CT and HT NPY enhanced the brain mRNA expression of HSP-70 and -90, as well as of NPYSRs-Y5, -Y6, and -Y7, but not -Y1, -Y2, and -Y4, under CT and HT A coinjection of an NPYSR-Y5 antagonist (CGP71683) and NPY (375 pmol) attenuated the NPY-induced hypothermia. Furthermore, central NPY decreased plasma glucose and triacylglycerol under CT and HT and kept plasma corticosterone and epinephrine lower under HT NPY increased plasma taurine and anserine concentrations. In conclusion, brain NPYSR-Y5 partially afforded protective thermotolerance in heat-exposed fasted chicks. The NPY-mediated reduction in plasma glucose and stress hormone levels and the increase in free amino acids in plasma further suggest that NPY might potentially play a role in minimizing heat stress in fasted chicks.
(© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)

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Keywords: NPY; Fasted chicks; HSP, NPY sub‐receptors; rectal temperature

0 (Blood Glucose)
0 (Heat-Shock Proteins)
0 (Naphthalenes)
0 (Neuropeptide Y)
0 (Pyrimidines)
0 (Receptors, Neuropeptide Y)
0 (neuropeptide Y5 receptor)
45B73P82A3 (CGP 71683 A)

Date Created: 20171207 Date Completed: 20180717 Latest Revision: 20210109

20250114

PMC5727273

10.14814/phy2.13511

29208684