Meox1 accelerates myocardial hypertrophic decompensation through Gata4.

Publisher: Oxford Journals Country of Publication: England NLM ID: 0077427 Publication Model: Print Cited Medium: Internet ISSN: 1755-3245 (Electronic) Linking ISSN: 00086363 NLM ISO Abbreviation: Cardiovasc Res Subsets: MEDLINE

Publication: 2008- : Oxford : Oxford Journals
Original Publication: London, British Medical Assn.

Ventricular Function, Left* ; Ventricular Remodeling*; Cardiomyopathy, Dilated/*metabolism ; Cardiomyopathy, Hypertrophic/*metabolism ; GATA4 Transcription Factor/*metabolism ; Heart Ventricles/*metabolism ; Homeodomain Proteins/*metabolism; Animals ; Cardiomyopathy, Dilated/genetics ; Cardiomyopathy, Dilated/pathology ; Cardiomyopathy, Dilated/physiopathology ; Cardiomyopathy, Hypertrophic/genetics ; Cardiomyopathy, Hypertrophic/pathology ; Cardiomyopathy, Hypertrophic/physiopathology ; Cell Line ; Disease Models, Animal ; Disease Progression ; GATA4 Transcription Factor/genetics ; Gene Expression Regulation ; Heart Ventricles/pathology ; Heart Ventricles/physiopathology ; Homeodomain Proteins/genetics ; Humans ; Mice, Inbred C57BL ; Mice, Knockout ; Signal Transduction ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcriptional Activation ; Troponin T/genetics ; Troponin T/metabolism

Aims: Pathological hypertrophy is the result of gene network regulation, which ultimately leads to adverse cardiac remodelling and heart failure (HF) and is accompanied by the reactivation of a 'foetal gene programme'. The Mesenchyme homeobox 1 (Meox1) gene is one of the foetal programme genes. Meox1 may play a role in embryonic development, but its regulation of pathological hypertrophy is not known. Therefore, this study investigated the effect of Meox1 on pathological hypertrophy, including familial and pressure overload-induced hypertrophy, and its potential mechanism of action.
Methods and Results: Meox1 expression was markedly down-regulated in the wild-type adult mouse heart with age, and expression was up-regulated in heart tissues from familial dilated cardiomyopathy (FDCM) mice of the cTnTR141W strain, familial hypertrophic cardiomyopathy (FHCM) mice of the cTnTR92Q strain, pressure overload-induced HF mice, and hypertrophic cardiomyopathy (HCM) patients. Echocardiography, histopathology, and hypertrophic molecular markers consistently demonstrated that Meox1 overexpression exacerbated the phenotypes in FHCM and in mice with thoracic aorta constriction (TAC), and that Meox1 knockdown improved the pathological changes. Gata4 was identified as a potential downstream target of Meox1 using digital gene expression (DGE) profiling, real-time PCR, and bioinformatics analysis. Promoter activity data and chromatin immunoprecipitation (ChIP) and Gata4 knockdown analyses indicated that Meox1 acted via activation of Gata4 transcription.
Conclusion: Meox1 accelerated decompensation via the downstream target Gata4, at least in part directly. Meox1 and other foetal programme genes form a highly interconnected network, which offers multiple therapeutic entry points to dampen the aberrant expression of foetal genes and pathological hypertrophy.
(Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2017. For permissions please email: journals.permissions@oup.com.)

Keywords: Cardiomyopathy; Foetal gene programme; Gene expression and regulation; Heart failure; Hypertrophy

0 (GATA4 Transcription Factor)
0 (Gata4 protein, mouse)
0 (Homeodomain Proteins)
0 (MEOX1 protein, human)
0 (Meox1 protein, mouse)
0 (Transcription Factors)
0 (Troponin T)

Familial dilated cardiomyopathy

Date Created: 20171121 Date Completed: 20190520 Latest Revision: 20190520

20231215

10.1093/cvr/cvx222

29155983