Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

The [Mo₆Cl 14 ] 2- Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • الموضوع:
      Antiviral Agents/*pharmacology ; Molybdenum/*chemistry ; Organometallic Compounds/*pharmacology ; Rotavirus/*drug effects; Cell Line ; Cell Survival/drug effects ; Erythrocytes/drug effects ; Hemolysis ; Hep G2 Cells ; Humans ; In Vitro Techniques ; Organometallic Compounds/chemistry ; Serum Albumin, Human/metabolism
    • نبذة مختصرة :
      The molybdenum cluster [Mo₆Cl 14 ] 2- is a fluorescent component with potential for use in cell labelling and pharmacology. Biological safety and antiviral properties of the cluster are as yet unknown. Here, we show the effect of acute exposition of human cells and red blood cells to the molybdenum cluster and its interaction with proteins and antiviral activity in vitro. We measured cell viability of HepG2 and EA.hy926 cell lines exposed to increasing concentrations of the cluster (0.1 to 250 µM), by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Hemolysis and morphological alterations of red blood cells, obtained from healthy donors, exposed to the cluster (10 to 200 µM) at 37 °C were analyzed. Furthermore, quenching of tryptophan residues of albumin was performed. Finally, plaque formation by rotavirus SA11 in MA104 cells treated with the cluster (100 to 300 µM) were analyzed. We found that all doses of the cluster showed similar cell viability, hemolysis, and morphology values, compared to control. Quenching of tryptophan residues of albumin suggests a protein-cluster complex formation. Finally, the cluster showed antiviral activity at 300 µM. These results indicate that the cluster [Mo₆Cl 14 ] 2- could be intravenously administered in animals at therapeutic doses for further in vivo studies and might be studied as an antiviral agent.
      Competing Interests: The authors declare no conflict of interest.
    • References:
      Dalton Trans. 2013 May 21;42(19):7224-32. (PMID: 23532319)
      Int J Pharm. 2008 Feb 4;348(1-2):125-36. (PMID: 17716842)
      Mutat Res. 1998 Jun 18;402(1-2):185-202. (PMID: 9675276)
      J Inorg Biochem. 2015 Mar;144:13-7. (PMID: 25575304)
      Inorg Chem. 2013 Jan 18;52(2):974-82. (PMID: 23301676)
      Inorg Chem. 2014 Sep 2;53(17):9006-13. (PMID: 25142977)
      Biomaterials. 2008 Aug-Sep;29(24-25):3469-76. (PMID: 18501424)
      Chem Commun (Camb). 2016 Oct 11;52(83):12286-12289. (PMID: 27722437)
      Sci Rep. 2015 Dec 03;5:17422. (PMID: 26632249)
      J Colloid Interface Sci. 2015 Nov 1;457:370-7. (PMID: 26196721)
      Inorg Chem. 2012 Dec 17;51(24):13289-302. (PMID: 23198846)
      Chimia (Aarau). 2015 Aug 19;69(7):442-446. (PMID: 28482977)
      Biochem Pharmacol. 2015 Jan 15;93(2):171-81. (PMID: 25449596)
      Anal Sci. 2007 Apr;23(4):429-33. (PMID: 17420547)
      Inorg Chem. 2008 Aug 18;47(16):7271-8. (PMID: 18646747)
      J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682)
      J Hazard Mater. 2012 Jun 15;219-220:111-8. (PMID: 22510541)
      Science. 2010 Jan 15;327(5963):331-4. (PMID: 19965379)
      Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 5;136 Pt C:1335-48. (PMID: 25456676)
      J Appl Toxicol. 1999 May-Jun;19(3):185-92. (PMID: 10362269)
      Anticancer Res. 2001 Jan-Feb;21(1A):93-102. (PMID: 11299795)
      Mol Oncol. 2012 Apr;6(2):140-6. (PMID: 22356776)
      Chem Biol Interact. 2015 Dec 5;242:61-70. (PMID: 26391003)
      J Inorg Biochem. 2008 Nov;102(11):1991-6. (PMID: 18783832)
      Environ Sci Technol. 2010 Mar 15;44(6):1962-7. (PMID: 20151631)
      Chem Rev. 1998 Feb 5;98(1):327-358. (PMID: 11851509)
      Toxicology. 2004 May 20;198(1-3):329-40. (PMID: 15138059)
      Sensors (Basel). 2010;10(1):428-55. (PMID: 22315549)
      Science. 2008 Jan 11;319(5860):210-3. (PMID: 18187657)
      J Proteome Res. 2017 Feb 3;16(2):689-697. (PMID: 27973853)
      Organometallics. 2017 May 8;36(9):1673-1676. (PMID: 29051683)
      J Mater Chem B. 2016 Jul 28;4(28):4839-4846. (PMID: 32263142)
      J Control Release. 2015 Aug 10;211:144-62. (PMID: 26055641)
      Nanomedicine (Lond). 2012 Sep;7(9):1355-64. (PMID: 22583573)
      J Arthroplasty. 2016 Sep;31(9):1910-5. (PMID: 26965589)
      Biol Trace Elem Res. 2016 Mar;170(1):237-44. (PMID: 26201682)
      Int J Mol Sci. 2015 Jan 13;16(1):1728-35. (PMID: 25590300)
      Int J Nanomedicine. 2011;6:1889-901. (PMID: 21931484)
      Curr Opin Gastroenterol. 2016 Jan;32(1):18-23. (PMID: 26574867)
    • Contributed Indexing:
      Keywords: albumin; cell viability; cluster; hemolysis; red blood cells; rotavirus
    • الرقم المعرف:
      0 (Antiviral Agents)
      0 (Organometallic Compounds)
      81AH48963U (Molybdenum)
      ZIF514RVZR (Serum Albumin, Human)
    • الموضوع:
      Date Created: 20170706 Date Completed: 20180329 Latest Revision: 20240331
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC6152029
    • الرقم المعرف:
      10.3390/molecules22071108
    • الرقم المعرف:
      28678175