Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 7608025 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2072 (Electronic) Linking ISSN: 00333158 NLM ISO Abbreviation: Psychopharmacology (Berl) Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag.

Analgesics, Opioid/*administration & dosage ; Inflammation Mediators/*metabolism ; Oxycodone/*administration & dosage ; Sequence Analysis, RNA/*methods ; Ventral Striatum/*drug effects ; Ventral Striatum/*metabolism; Age Factors ; Animals ; Dopamine ; Gene Expression/drug effects ; Gene Expression/physiology ; Inflammation/genetics ; Inflammation/immunology ; Inflammation/metabolism ; Inflammation Mediators/immunology ; Male ; Mice ; Mice, Inbred C57BL ; Self Administration ; Ventral Striatum/immunology

Introduction: Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone's impact on diverse gene systems in the brain.
Objectives: The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.
Method: Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.
Result: We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.
Conclusion: This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone.

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R01 DA029147 United States DA NIDA NIH HHS

Keywords: C57BL/6J mice; Inflammation and immune-related genes; Oxycodone self-administration; RNA sequencing

0 (Analgesics, Opioid)
0 (Inflammation Mediators)
CD35PMG570 (Oxycodone)
VTD58H1Z2X (Dopamine)

Date Created: 20170628 Date Completed: 20180306 Latest Revision: 20220330

20240829

PMC5826641

10.1007/s00213-017-4657-y

28653080