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Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure.

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  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      Cells, Immobilized*/metabolism ; Cells, Immobilized*/pathology ; Hepatocytes*/metabolism ; Hepatocytes*/pathology ; Liver Failure, Acute*/metabolism ; Liver Failure, Acute*/pathology ; Liver Failure, Acute*/therapy ; Liver, Artificial* ; Spheroids, Cellular*/metabolism ; Spheroids, Cellular*/pathology; Animals ; Disease Models, Animal ; Male ; Swine
    • نبذة مختصرة :
      Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three groups. The control group consisted of treatment naïve pigs (n = 5), blank group consisted of pigs that were attached to the BAL system not containing hepatocytes for 12 hours (n = 5) and BAL group consisted of pigs that were attached to the BAL containing hepatocytes for 12 hours (n = 5). Increase in serum ammonia levels were significantly greater in the blank group (P < 0.01) and control group (P < 0.01), compared to the BAL group during the treatment period. Increase in ICP was significantly greater in the control group compared to the BAL group (P = 0.01). Survival was significantly prolonged in the BAL group compared to the blank group (P = 0.03). A BAL system with a bioreactor containing hepatocyte spheroids showed effective clearance of serum ammonia, preservation of renal function and delayed ICP increase in a porcine model of ALF.
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    • الموضوع:
      Date Created: 20170621 Date Completed: 20181219 Latest Revision: 20220311
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC5476639
    • الرقم المعرف:
      10.1038/s41598-017-03424-2
    • الرقم المعرف:
      28630420