Effect of Maternal Obesity on Fetal Growth and Expression of Placental Fatty Acid Transporters.

Publisher: Galenos Yayınevi Country of Publication: Turkey NLM ID: 101519456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1308-5735 (Electronic) NLM ISO Abbreviation: J Clin Res Pediatr Endocrinol Subsets: MEDLINE

Publication: Istanbul : Galenos Yayınevi
Original Publication: Istanbul : Turkish Pediatric Endocrinology and Diabetes Society

Obesity*/genetics ; Obesity*/physiopathology ; Pregnancy Complications*/genetics ; Pregnancy Complications*/physiopathology; Fatty Acid Transport Proteins/*genetics ; Fetal Development/*physiology ; Placenta/*metabolism; Animals ; Diet, High-Fat ; Fatty Acid Transport Proteins/metabolism ; Fatty Acids/metabolism ; Female ; Male ; Maternal Nutritional Physiological Phenomena ; Pregnancy ; Rats ; Rats, Sprague-Dawley

Objective: To explore the effects of maternal high-fat (HF) diet-induced obesity on fetal growth and the expression of placental nutrient transporters.
Methods: Maternal obesity was established in rats by 8 weeks of pre-pregnancy fed HF diet, while rats in the control group were fed normal (CON) diet. Diet-induced obesity (DIO) rats and diet-induced obesity-resistant (DIR) rats were selected according to body weight gain over this period. After copulation, the CON rats were divided into two groups: switched to HF diet (CON-HF group) or maintained on the CON diet (CON-CON group). The DIO rats and DIR rats were maintained on the HF diet throughout pregnancy. Pregnant rats were euthanized at day 21 gestation, fetal and placental weights were recorded, and placental tissue was collected. Reverse transcription-polymerase chain reaction was used to determine mRNA expression of placental nutrient transporters. Protein expression was determined by Western blot.
Results: Average fetal weight of DIO dams was reduced by 6.9%, and the placentas of CON-HF and DIO dams were significantly heavier than the placentas of CON-CON and DIR dams at day 21 of gestation (p<0.05). The fetal/placental weight ratio of DIO dams was significantly reduced compared with the fetal/placental weight ratio of CON-CON dams (p<0.05). The mRNA expression of GLUT-1 and SNAT-2 were not significantly different between groups. The mRNA and protein expression levels of CD36, FATP-1, and FATP-4 in DIO dams were decreased significantly (p<0.05).
Conclusion: Maternal obesity induced by a HF diet led to intrauterine growth retardation and down-regulated the expression of placental fatty acid transporters.

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Keywords: Obesity; high-fat diet intrauterine growth.; nutrient transport; placenta

0 (Fatty Acid Transport Proteins)
0 (Fatty Acids)

Date Created: 20170608 Date Completed: 20180206 Latest Revision: 20181113

20250114

PMC5785635

10.4274/jcrpe.4510

28588000