Pre-harvest aflatoxins and Aspergillus flavus contamination in variable germplasms of red chillies from Kunri, Pakistan.

Publisher: Springer-Verlag Berlin Country of Publication: Germany NLM ID: 8807334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1867-1632 (Electronic) Linking ISSN: 01787888 NLM ISO Abbreviation: Mycotoxin Res Subsets: MEDLINE

Publication: Heidelberg : Springer-Verlag Berlin
Original Publication: Mainz, Federal Republic of Germany : Hans W. Schmidt, 1985-

Food Contamination*; Aflatoxins/*analysis ; Aspergillus flavus/*isolation & purification ; Capsicum/*chemistry ; Capsicum/*microbiology; Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Cluster Analysis ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; DNA, Ribosomal Spacer/chemistry ; DNA, Ribosomal Spacer/genetics ; Microbiological Techniques ; Pakistan ; Phylogeny ; Polymerase Chain Reaction ; Sequence Analysis, DNA

Various cultivars of red chilli were collected from a small town named Kunri, located in the province Sindh, Pakistan. This town is a hub of red chilli production in Asia. A total of 69 samples belonging to 6 cultivars were obtained and analysed for the occurrence of aflatoxins and Aspergillus flavus, to explore the potential of resistant and susceptible germplasm. Aflatoxins were detected by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), while A. flavus was isolated and identified using agar plate, blotter paper, deep freezing and dilution techniques. Molecular characterization using internal transcribed spacer (ITS) 1/4 and A. flavus specific FL1-F/R primers confirmed the identity of A. flavus. The data revealed that 67 and 75% samples contaminated with aflatoxin B 1 (AFB 1 ) and with A. flavus, respectively. A highly susceptible chilli cultivar was 'Nagina', showing 78.8% frequency of total aflatoxins (1.2-600 μg/kg) and a mean of 87.7 μg/kg for AFB 1 and 121.9 μg/kg for total aflatoxins. A. flavus was detected with 93% frequency and 2.14 × 10 ⁴ colony forming units. In contrast, cultivars 'Kunri' and 'Drooping Type' were found to be resistant, with low levels of aflatoxins and fungal counts. The study was conducted for the first time to explore two potential cultivars that were less susceptible towards A. flavus and aflatoxin contamination. These cultivars could be preferably cultivated and thereby boost Pakistan's chilli production.

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Keywords: Aflatoxin; Aspergillus flavus; Capsicum annuum; Cultivars; Resistance; Susceptible

0 (Aflatoxins)
0 (DNA, Fungal)
0 (DNA, Ribosomal Spacer)

Date Created: 20170407 Date Completed: 20170503 Latest Revision: 20201209

20240829

10.1007/s12550-017-0274-1

28382601