Better understanding of homologous recombination through a 12-week laboratory course for undergraduates majoring in biotechnology.

Publisher: John Wiley & sons Country of Publication: United States NLM ID: 100970605 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-3429 (Electronic) Linking ISSN: 14708175 NLM ISO Abbreviation: Biochem Mol Biol Educ Subsets: MEDLINE

Publication: 2002- : Hoboken, NJ : John Wiley & sons
Original Publication: Oxford, UK : Elsevier, c2000-

Homologous Recombination*; Biotechnology/*education ; Education, Medical, Undergraduate/*methods ; Educational Measurement/*statistics & numerical data ; Molecular Biology/*education; Humans ; Learning ; Mutation ; Plasmids/chemistry ; Plasmids/metabolism ; Streptococcus suis/genetics ; Streptococcus suis/metabolism ; Streptococcus suis/ultrastructure ; Students ; Surveys and Questionnaires

Homologous recombination, a central concept in biology, is defined as the exchange of DNA strands between two similar or identical nucleotide sequences. Unfortunately, undergraduate students majoring in biotechnology often experience difficulties in understanding the molecular basis of homologous recombination. In this study, we developed and implemented a 12-week laboratory course for biotechnology undergraduates in which gene targeting in Streptococcus suis was used to facilitate their understanding of the basic concept and process of homologous recombination. Students worked in teams of two to select a gene of interest to create a knockout mutant using methods that relied on homologous recombination. By integrating abstract knowledge and practice in the process of scientific research, students gained hands-on experience in molecular biology techniques while learning about the principle and process of homologous recombination. The learning outcomes and survey-based assessment demonstrated that students substantially enhanced their understanding of how homologous recombination could be used to study gene function. Overall, the course was very effective for helping biotechnology undergraduates learn the theory and application of homologous recombination, while also yielding positive effects in developing confidence and scientific skills for future work in research. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(4):329-335, 2017.
(© 2017 The International Union of Biochemistry and Molecular Biology.)

Keywords: Homologous recombination; biotechnology; laboratory course; undergraduate students

Date Created: 20170318 Date Completed: 20170720 Latest Revision: 20170720

20221213

10.1002/bmb.21043

28314074