pOsNAR2.1:OsNAR2.1 expression enhances nitrogen uptake efficiency and grain yield in transgenic rice plants.

Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE

Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists
Original Publication: [Oxford] : Blackwell Pub., c2003-

Anion Transport Proteins/*genetics ; Nitrates/*metabolism ; Nitrogen/*metabolism ; Oryza/*genetics ; Plants, Genetically Modified/*metabolism; Ammonium Compounds/metabolism ; Anion Transport Proteins/metabolism ; Biomass ; Fertilizers ; Nitrate Transporters ; Oryza/growth & development ; Oryza/metabolism ; Plants, Genetically Modified/growth & development ; Promoter Regions, Genetic ; Seedlings/growth & development

The nitrate (NO3-) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transferred another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enhanced OsNAR2.1 expression level, compared with wild type (WT), and ¹⁵ N influx in roots increased 21%-32% in response to 0.2 mm and 2.5 mm 15NO3- and 1.25 mm ¹⁵ NH 4 ¹⁵ NO 3 . Under these three N conditions, the biomass of the pOsNAR2.1:OsNAR2.1 transgenic lines increased 143%, 129% and 51%, and total N content increased 161%, 242% and 69%, respectively, compared to WT. Furthermore in field experiments we found the grain yield, agricultural nitrogen use efficiency (ANUE), and dry matter transfer of pOsNAR2.1:OsNAR2.1 plants increased by about 21%, 22% and 21%, compared to WT. We also compared the phenotypes of pOsNAR2.1:OsNAR2.1 and pOsNAR2.1:OsNRT2.1 transgenic lines in the field, found that postanthesis N uptake differed significantly between them, and in comparison with the WT. Postanthesis N uptake (PANU) increased approximately 39% and 85%, in the pOsNAR2.1:OsNAR2.1 and pOsNAR2.1:OsNRT2.1 transgenic lines, respectively, possibly because OsNRT2.1 expression was less in the pOsNAR2.1:OsNAR2.1 lines than in the pOsNAR2.1:OsNRT2.1 lines during the late growth stage. These results show that rice NO 3 ^- uptake, yield and NUE were improved by increased OsNAR2.1 expression via its native promoter.
(© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)

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Keywords: Oryza sativa; OsNAR2.1; Nitrogen uptake efficiency; OsNAR2.1 promoter

0 (Ammonium Compounds)
0 (Anion Transport Proteins)
0 (Fertilizers)
0 (Nitrate Transporters)
0 (Nitrates)
N762921K75 (Nitrogen)

Date Created: 20170223 Date Completed: 20180305 Latest Revision: 20240326

20240326

PMC5595721

10.1111/pbi.12714

28226420