Functional identification of apple MdJAZ2 in Arabidopsis with reduced JA-sensitivity and increased stress tolerance.

Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer, 1981-

Genes, Plant*; Adaptation, Physiological/*genetics ; Arabidopsis/*physiology ; Cyclopentanes/*pharmacology ; Malus/*genetics ; Oxylipins/*pharmacology ; Plant Proteins/*metabolism ; Stress, Physiological/*genetics; Adaptation, Physiological/drug effects ; Amino Acid Sequence ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Droughts ; Gene Expression Regulation, Plant/drug effects ; Plant Proteins/genetics ; Plants, Genetically Modified ; Protein Binding/drug effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sodium Chloride/pharmacology ; Stress, Physiological/drug effects ; Subcellular Fractions/drug effects ; Subcellular Fractions/metabolism

Key Message: Here, we report the decrease of JA-sensitivity and enhancement of tolerance to salt and PEG stresses in Arabidopsis overexpressing apple MdJAZ2. As signalling molecules, jasmonates (JAs) play significant roles in plant development and stress responses. JAZ proteins are the targets of the SCF ^COI1 complex and act as the negative regulators in JA signalling pathway. However, there are no reports regarding the biological function of apple JAZ genes. In this study, one JAZ gene, MdJAZ2 from apple, was functionally characterized in detail. The expression of MdJAZ2 was up-regulated by MeJA and wounding treatments. MdJAZ2-GFP fusion protein was observed in nucleus in transient expression assay. Yeast two-hybrid and bimolecular fluorescence complementation assays revealed that MdJAZ2 could form homo- and heteromers, and also interact with F-box protein MdCOI1. Overexpression of MdJAZ2 conferred impaired JA-sensitivity in transgenic Arabidopsis, including JA-mediated root growth inhibition, susceptibility to the bacterial pathogen Pst DC3000, and the expression of JA response genes. Additionally, MdJAZ2 overexpression also improved tolerance to NaCl and PEG treatments in transgenic Arabidopsis. Together, our findings suggest that apple MdJAZ2 was not only involved in the JA response but also played roles in stress tolerance.

Annu Rev Plant Biol. 2008;59:41-66. (PMID: 18031220)
Plant Cell. 2011 Feb;23(2):701-15. (PMID: 21335373)
Plant Cell. 2011 Sep;23(9):3089-100. (PMID: 21963667)
Biosci Biotechnol Biochem. 2012;76(11):2129-34. (PMID: 23132589)
Phytochemistry. 2009 Sep;70(13-14):1547-59. (PMID: 19800644)
Plant J. 2011 Mar;65(6):907-21. (PMID: 21332845)
Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079)
PLoS One. 2012;7(9):e44465. (PMID: 22984514)
PLoS One. 2015 Mar 25;10(3):e0121824. (PMID: 25807368)
Arabidopsis Book. 2002;1:e0039. (PMID: 22303207)
Proc Natl Acad Sci U S A. 2008 May 13;105(19):7100-5. (PMID: 18458331)
Trends Plant Sci. 2007 Jun;12(6):239-44. (PMID: 17499004)
Nature. 2010 Nov 18;468(7322):400-5. (PMID: 20927106)
Vitam Horm. 2005;72:431-56. (PMID: 16492478)
Plant J. 2009 Jul;59(1):77-87. (PMID: 19309455)
Trends Plant Sci. 2008 Feb;13(2):66-71. (PMID: 18261950)
Genomics. 2011 Aug;98(2):128-36. (PMID: 21616136)
Dev Cell. 2010 Dec 14;19(6):884-94. (PMID: 21145503)
Nature. 2007 Aug 9;448(7154):666-71. (PMID: 17637675)
Plant Cell. 2007 Jul;19(7):2225-45. (PMID: 17616737)
Plant Cell Rep. 2013 Feb;32(2):263-72. (PMID: 23090726)
Ann Bot. 2013 Jun;111(6):1021-58. (PMID: 23558912)
Science. 1998 May 15;280(5366):1091-4. (PMID: 9582125)
Plant Cell. 2011 Mar;23(3):1000-13. (PMID: 21447791)
Plant J. 2005 May;42(4):514-24. (PMID: 15860010)
Nat Chem Biol. 2009 May;5(5):344-50. (PMID: 19349968)
Plant Physiol. 2011 Feb;155(2):751-64. (PMID: 21173027)
Plant Cell. 2009 Jan;21(1):131-45. (PMID: 19151223)
Plant Cell. 2002 Aug;14 (8):1919-35. (PMID: 12172031)
Nature. 2010 Apr 1;464(7289):788-91. (PMID: 20360743)
Nature. 2007 Aug 9;448(7154):661-5. (PMID: 17637677)
Plant Cell. 2013 Feb;25(2):486-98. (PMID: 23386265)
Plant Mol Biol. 2009 Oct;71(3):291-305. (PMID: 19618278)
Plant Cell Rep. 2008 Jan;27(1):125-35. (PMID: 17786451)
Plant Cell. 2007 Aug;19(8):2470-83. (PMID: 17675405)
Nature. 2007 Aug 9;448(7154):659-60. (PMID: 17687317)
Mol Biol Evol. 2007 Aug;24(8):1596-9. (PMID: 17488738)
Plant Cell Physiol. 2015 Apr;56(4):650-62. (PMID: 25527830)
Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12539-44. (PMID: 21737749)
Plant J. 2008 Sep;55(6):979-88. (PMID: 18547396)
Plant Cell. 2011 May;23(5):1795-814. (PMID: 21551388)
Plant Physiol. 2008 Mar;146(3):832-8. (PMID: 18316637)
J Exp Bot. 2016 Apr;67(8):2367-86. (PMID: 26896849)
Front Plant Sci. 2012 Mar 08;3:41. (PMID: 22629274)

Keywords: Apple; JAZ protein; Jasmonate response; Protein interaction; Stress response; Transgenic Arabidopsis

0 (Cyclopentanes)
0 (Oxylipins)
0 (Plant Proteins)
0 (RNA, Messenger)
451W47IQ8X (Sodium Chloride)
6RI5N05OWW (jasmonic acid)

Date Created: 20161116 Date Completed: 20170327 Latest Revision: 20220408

20231215

10.1007/s00299-016-2077-9

27844101