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An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2825 (Electronic) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE
    • بيانات النشر:
      Publication: Hoboken, N.J. : Wiley
      Original Publication: New York : Alan R. Liss, Inc., c1987-
    • الموضوع:
      Diabetes Mellitus, Type 2/*genetics ; Perilipin-1/*genetics ; Polymerase Chain Reaction/*methods ; Polymorphism, Single Nucleotide/*genetics; China/ethnology ; Female ; Gene Frequency ; Genotype ; Humans ; Male
    • نبذة مختصرة :
      Background: In recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs2289487 of PLIN1 gene.
      Methods: A new restriction enzyme site was created through created restriction site PCR. In the forward primer, a deoxynucleotide A was substituted with C, and after PCR a new restriction enzyme site for BstUI was introduced into the PCR products. A total of 108 samples from Han Chinese were tested to evaluate this new method.
      Results: Allele frequencies in the Asian population were 0.326 for allele A and 0.674 for allele G, and the genotype frequencies were 12.8% for AA, 39.5% for AG, and 47.7% for GG.
      Conclusion: The PCR-RFLP with new site for detecting the SNP of rs2289487 is an improved method with low cost and high accuracy.
      (© 2016 Wiley Periodicals, Inc.)
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    • Contributed Indexing:
      Keywords: PLIN1; PCR-RFLP; created restriction site; rs2289487; single-nucleotide polymorphism
    • الرقم المعرف:
      0 (PLIN1 protein, human)
      0 (Perilipin-1)
    • الموضوع:
      Date Created: 20160910 Date Completed: 20170406 Latest Revision: 20191114
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC6806717
    • الرقم المعرف:
      10.1002/jcla.21968
    • الرقم المعرف:
      27611592