Juvenile Male Rats Exposed to a Low-Dose Mixture of Twenty-Seven Environmental Chemicals Display Adverse Health Effects.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Dioxins/*toxicity ; Environmental Pollutants/*toxicity ; Heterocyclic Compounds/*toxicity ; Metals/*toxicity ; Pesticides/*toxicity ; Phthalic Acids/*toxicity ; Polychlorinated Biphenyls/*toxicity; Animals ; Body Weight/drug effects ; Dioxins/blood ; Dioxins/urine ; Environmental Pollutants/blood ; Environmental Pollutants/urine ; Gene Expression Profiling ; Gene Expression Regulation ; Heterocyclic Compounds/blood ; Heterocyclic Compounds/urine ; Humans ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Liver/drug effects ; Liver/metabolism ; Liver/pathology ; Male ; Metabolome ; Metals/blood ; Metals/urine ; Pesticides/blood ; Pesticides/urine ; Phospholipids/blood ; Phospholipids/urine ; Phthalic Acids/blood ; Phthalic Acids/urine ; Polychlorinated Biphenyls/blood ; Polychlorinated Biphenyls/urine ; Rats ; Spleen/drug effects ; Spleen/metabolism ; Spleen/pathology

Humans are exposed to a large number of environmental chemicals in their daily life, many of which are readily detectable in blood or urine. It remains uncertain if these chemicals can cause adverse health effects when present together at low doses. In this study we have tested whether a mixture of 27 chemicals administered orally to juvenile male rats for three months could leave a pathophysiological footprint. The mixture contained metals, perfluorinated compounds, PCB, dioxins, pesticides, heterocyclic amines, phthalate, PAHs and others, with a combined dose of 0.16 (Low dose), 0.47 (Mid dose) or 1.6 (High dose) mg/kg bw/day. The lowest dose was designed with the aim of obtaining plasma or urine concentrations in rats at levels approaching those observed in humans. Some single congeners were administered at doses representative of combined doses for chemical groups. With this baseline, we found effects on weight, histology and gene expression in the liver, as well as changes to the blood plasma metabolome in all exposure groups, including low-dose. Additional adverse effects were observed in the higher dosed groups, including enlarged kidneys and alterations to the metabolome. No significant effects on reproductive parameters were observed.
Competing Interests: The authors have declared that no competing interests exist.

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0 (Dioxins)
0 (Environmental Pollutants)
0 (Heterocyclic Compounds)
0 (Metals)
0 (Pesticides)
0 (Phospholipids)
0 (Phthalic Acids)
6O7F7IX66E (phthalic acid)
DFC2HB4I0K (Polychlorinated Biphenyls)

Date Created: 20160907 Date Completed: 20170802 Latest Revision: 20190212

20231215

PMC5012700

10.1371/journal.pone.0162027

27598887