In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Magnetite Nanoparticles*; Antibodies/*pharmacology ; Brain Ischemia/*diagnostic imaging ; Cell Tracking/*methods ; Contrast Media/*pharmacology ; Fucosyltransferases/*antagonists & inhibitors ; Magnetic Resonance Imaging/*methods ; Neural Stem Cells/*pathology ; Stroke/*diagnostic imaging; Animals ; Brain Ischemia/metabolism ; Mice ; Neural Stem Cells/metabolism ; Stroke/pathology

Acute ischemic stroke remains a leading cause of death and disability. Endogenous neurogenesis enhanced via activation of neural stem cells (NSCs) could be a promising method for stroke treatment. In vivo targeted tracking is highly desirable for monitoring the dynamics of endogenous NSCs in stroke. Previously, we have successfully realized in vivo targeted MR imaging of endogenous NSCs in normal adult mice brains by using anti-CD15 antibody-conjugated superparamagnetic iron oxide nanoparticles (anti-CD15-SPIONs) as the molecular probe. Herein, we explore the performance of this molecular probe in targeted in vivo tracking of activated endogenous NSCs in ischemic stroke. Our study showed that intraventricular injection of anti-CD15-SPIONs could label activated endogenous NSCs in situ seven days after ischemic stroke, which were detected as enlarged areas of hypo-intense signals on MR imaging at 7.0 T. The treatment of cytosine arabinosine could inhibit the activation of endogenous NSCs, which was featured by the disappearance of areas of hypo-intense signals on MR imaging. Using anti-CD15-SPIONs as imaging probes, the dynamic process of activation of endogenous NSCs could be readily monitored by in vivo MR imaging. This targeted imaging strategy would be of great benefit to develop a new therapeutic strategy utilizing endogenous NSCs for ischemic stroke.

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Keywords: adult; iron oxide nanoparticles; ischemic stroke; magnetic resonance imaging; monoclonal anit-CD15 antibody; neural stem cells

0 (Antibodies)
0 (Contrast Media)
0 (Magnetite Nanoparticles)
EC 2.4.1.- (Fucosyltransferases)
EC 2.4.1.- (Fut4 protein, mouse)

Date Created: 20160903 Date Completed: 20170417 Latest Revision: 20200225

20240829

PMC6273863

10.3390/molecules21091143

27589699