Overexpression of molecular chaperons GRP78 and GRP94 in CD44(hi)/CD24(lo) breast cancer stem cells.

Publisher: Tabriz University of Medical Sciences Country of Publication: Iran NLM ID: 101558125 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2228-5652 (Print) Linking ISSN: 22285652 NLM ISO Abbreviation: Bioimpacts Subsets: PubMed not MEDLINE

Original Publication: Tabriz : Tabriz University of Medical Sciences

Introduction: Breast cancer stem cell with CD44(hi)/CD24(lo) phonotype is described having stem cell properties and represented as the main driving factor in breast cancer initiation, growth, metastasis and low response to anti-cancer agents. Glucoseregulated proteins (GRPs) are heat shock protein family chaperons that are charged with regulation of protein machinery and modulation of endoplasmic reticulum homeostasis whose important roles in stem cell development and invasion of various cancers have been demonstrated. Here, we investigated the expression levels of GRP78 and GRP94 in CD44(hi)/CD24(lo) phenotype breast cancer stem cells (BCSCs).
Methods: MCF7, T-47D and MDA-MB-231 breast cancer cell lines were used. CD44(hi)/CD24(lo) phenotype cell population were analyzed and sorted by fluorescence-activated cell sorting (FACS). Transcriptional and translational expression of GRP78 and GRP94 were investigated by western blotting and quantitative real time PCR.
Results: RESULTS showed different proportion of CD44(hi)/CD24(lo) phenotype cell population in their original bulk cells. The ranking of the cell lines in terms of CD44(hi)/CD24(lo) phenotype cell population was as MCF7Conclusion: Our results show a relationship between overexpression of GRP78 and GRP94 and exhibiting CD44hi/CD24lo phenotype in breast cancer cells. We conclude that upregulation of GRPs may be an important factor in the emergence of CD44hi/CD24lo phenotype BCSCs features.

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Keywords: Breast cancer; Cancer stem cell; GRP78; GRP94; Overexpression

Date Created: 20160816 Date Completed: 20160815 Latest Revision: 20200930

20250114

PMC4981248

10.15171/bi.2016.16

27525228