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Flow Preconditioning of Endothelial Cells on Collagen-Immobilized Silicone Fibers Enhances Cell Retention and Antithrombotic Function.

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  • معلومة اضافية
    • المصدر:
      Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 7802778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-1594 (Electronic) Linking ISSN: 0160564X NLM ISO Abbreviation: Artif Organs Subsets: MEDLINE
    • بيانات النشر:
      Publication: Cambridge, MA : Wiley-Blackwell
      Original Publication: Cleveland, International Society for Artificial Organs.
    • الموضوع:
      Bioartificial Organs*; Coated Materials, Biocompatible/*chemistry ; Collagen/*chemistry ; Endothelial Cells/*cytology ; Silicones/*chemistry ; Tissue Engineering/*instrumentation; Cell Adhesion ; Endothelial Cells/metabolism ; Endothelium, Vascular/cytology ; Endothelium, Vascular/metabolism ; Equipment Design ; Human Umbilical Vein Endothelial Cells ; Humans ; Hydrodynamics ; Immobilized Proteins/chemistry ; Lung/blood supply ; Lung/cytology ; Lung/physiology ; Materials Testing ; Nitric Oxide/metabolism ; Prostaglandins/metabolism ; Stress, Mechanical ; Tissue Engineering/methods
    • نبذة مختصرة :
      Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm 2 , 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm 2 , 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I 2 (1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.
      (© 2016 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.)
    • Contributed Indexing:
      Keywords: Antithrombotic function; Biohybrid artificial lung; Collagen immobilization; Endothelialization; Finite element modeling; Fluid shear stress
    • الرقم المعرف:
      0 (Coated Materials, Biocompatible)
      0 (Immobilized Proteins)
      0 (Prostaglandins)
      0 (Silicones)
      31C4KY9ESH (Nitric Oxide)
      9007-34-5 (Collagen)
    • الموضوع:
      Date Created: 20160716 Date Completed: 20171012 Latest Revision: 20171012
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1111/aor.12759
    • الرقم المعرف:
      27418522