Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.

Publisher: Munksgaard Country of Publication: Denmark NLM ID: 9301549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0625 (Electronic) Linking ISSN: 09066705 NLM ISO Abbreviation: Exp Dermatol Subsets: MEDLINE

Original Publication: Copenhagen : Munksgaard, c1992-

Antimicrobial Cationic Peptides/*metabolism ; Kallikreins/*metabolism ; Keratinocytes/*radiation effects ; Rosacea/*radiotherapy ; Toll-Like Receptor 2/*metabolism; Animals ; Cells, Cultured ; Humans ; Interleukin-8/metabolism ; Keratinocytes/metabolism ; Mice, Inbred BALB C ; Receptor, PAR-2/metabolism ; Receptors, Calcitriol/metabolism ; Rosacea/metabolism ; Cathelicidins

Cathelicidin (LL-37), Toll-like receptor 2 (TLR-2) and kallikreins (KLKs) are key inflammatory mediators in rosacea. Laser or light-based devices have been successfully used for rosacea. We investigated the effects of light-emitting diodes (LEDs) on LL-37, KLKs, TLR-2 and protease activity in cultured normal human epidermal keratinocytes (NHEKs) and rosacea-like mouse skin (RLMS). LL-37, KLK5, KLK7 and vitamin D receptor were induced by 1α, 25-dihydroxyvitamin D 3 (VD 3 ) and TLR-2 by Ad-CMV transfection in cultured NHEKs. NHEKs were subjected to LED irradiation at differing wavelengths (480-940 nm) and fluences (1-40 J/cm ² ). Inflammatory mediators were analysed with RT-PCR and real-time PCR and protease activity analysis and immunocytofluorescence staining were performed for NHEKs. Changes in RLMS induced by LL-37 peptide were evaluated with real-time PCR, immunohistochemical staining and enzyme-linked immunosorbent assay. In NHEKs, LED at 630 and 940 nm significantly attenuated LL37, KLK5 and TLR-2 mRNA expressions. Protease activity was significantly suppressed at 630, 850 and 940 nm. In the RLMS, LL-37, KLK5 and PAR-2 mRNA expressions significantly decreased at 24 and 48 hours after LED irradiation was performed three times at 630 and 940 nm. mCAMP and IL-8 protein levels and protease activity after LED irradiation were lower than those in RLMS control groups. LED at 630 and 940 nm downregulated TLR-2, KLK5 and LL-37 expressions and protease activity in NHEK and RLMS. Thus, LEDs may be promising for rosacea treatment. However, clinical trials are required for further study.
(© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Keywords: cathelicidin; kallikrein; light-emitting diode; rosacea

0 (Antimicrobial Cationic Peptides)
0 (Interleukin-8)
0 (Receptor, PAR-2)
0 (Receptors, Calcitriol)
0 (Toll-Like Receptor 2)
EC 3.4.21.- (Kallikreins)
0 (Cathelicidins)

Date Created: 20160618 Date Completed: 20180126 Latest Revision: 20221207

20231215

10.1111/exd.13133

27315464