Cloning, expression and antioxidant activity of a novel collagen from Pelodiscus sinensis.

Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE

Publication: 2005- : Berlin : Springer
Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990-

Antioxidants/*chemistry ; Collagen/*chemistry ; Collagen/*genetics ; Collagen/*metabolism ; Turtles/*genetics; Animals ; Biphenyl Compounds/chemistry ; Cloning, Molecular ; Collagen/biosynthesis ; Escherichia coli/genetics ; Free Radicals/chemistry ; Hydroxyl Radical/chemistry ; Phylogeny ; Picrates/chemistry ; Sequence Analysis, Protein

Collagen is the main structural protein of various connective tissues in animals and naturally plays an important role within the body. It is increasingly used within certain areas, such as medicine, citology and cosmetology. The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species rich in collagen. In this study, a novel collagen gene fragment of 756 bp, which encodes 252 deduced amino acid residues, including 25 conserved Gly-X-Y motifs, was cloned from a soft-shelled turtle. Recombinant soft-shelled turtle collagen (rSTC) was stably expressed in Escherichia coli Rosetta and purified by His GraviTrap affinity columns. The antioxidant activities of rSTC were measured using hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The results showed that rSTC quenched the free radicals in a dose-dependent manner. The hydroxyl radical scavenging activity (HRSA) of rSTC was 98.9 % at a concentration of 3 mg/mL. At a concentration of 5 mg/mL, rSTC exhibited a DPPH radical scavenging activity of 32.7 %. At the tested concentrations, rSTC exhibited higher HRSA and lower DPPH radical scavenging activity.

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Keywords: Antioxidant activity; Cloning; Collagen; Expression; Pelodiscus sinensis

0 (Antioxidants)
0 (Biphenyl Compounds)
0 (Free Radicals)
0 (Picrates)
3352-57-6 (Hydroxyl Radical)
9007-34-5 (Collagen)
DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)

Date Created: 20160428 Date Completed: 20170413 Latest Revision: 20181113

20240829

10.1007/s11274-016-2057-9

27116966