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Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus.

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  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, [2001-
    • الموضوع:
      Phagocytosis*; Brucella abortus/*pathogenicity ; Macrophages/*immunology ; Macrophages/*microbiology ; Platelet Membrane Glycoproteins/*metabolism ; Receptors, G-Protein-Coupled/*metabolism; Actin Cytoskeleton/metabolism ; Animals ; Brucella abortus/metabolism ; Brucellosis/metabolism ; Brucellosis/microbiology ; Endosomes/metabolism ; Host-Pathogen Interactions ; Janus Kinase 2/metabolism ; Macrophages/enzymology ; Mice ; RAW 264.7 Cells ; Signal Transduction
    • نبذة مختصرة :
      Background: Brucella abortus is an intracellular pathogen which can infect and persist in host cells through multiple interactions. Above all, its interaction to host cell receptor is important to understand the pathogenic mechanisms of B. abortus. Accordingly, we demonstrated that platelet-activating factor receptor (PAFR) affects host cell response against B. abortus infection.
      Results: First of all, B. abortus infection to macrophage induces secretion of platelet-activating factor (PAF), which is a PAFR agonist. The stimulation of PAFR by PAF remarkably increases B. abortus uptake into macrophages. It induces Janus kinase 2 (JAK2) and p38α phosphorylation, indicating that PAFR-mediated activation of JAK2 signaling leads to enhanced uptake of B. abortus. Moreover, the dynamics of F-actin polymerization revealed that PAFR-mediated B. abortus uptake is related with the reorganization of F-actin and JAK2. Upon B. abortus phagocytosis, reduced PAFR in the membrane and subsequently increased levels of PAFR colocalization with endosomes were observed which indicate that B. abortus uptake into macrophages allowed PAFR trafficking to endosomes.
      Conclusions: This study demonstrated that PAFR has a compelling involvement in B. abortus uptake as a promoter of phagocytosis, which is associated with JAK2 activation. Thus, our findings establish a novel insight into a receptor-related phagocytic mechanism of B. abortus.
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    • Contributed Indexing:
      Keywords: B. abortus; JAK2; Phagocytosis; Platelet-activating factor receptor
    • الرقم المعرف:
      0 (Platelet Membrane Glycoproteins)
      0 (Receptors, G-Protein-Coupled)
      0 (platelet activating factor receptor)
      EC 2.7.10.2 (Jak2 protein, mouse)
      EC 2.7.10.2 (Janus Kinase 2)
    • الموضوع:
      Date Created: 20160422 Date Completed: 20161213 Latest Revision: 20181113
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC4839150
    • الرقم المعرف:
      10.1186/s12866-016-0685-8
    • الرقم المعرف:
      27098179