Evaluation of five conventional and molecular approaches for diagnosis of cryptococcal meningitis in non-HIV-infected patients.

Publisher: Grosse Verlag Country of Publication: Germany NLM ID: 8805008 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0507 (Electronic) Linking ISSN: 09337407 NLM ISO Abbreviation: Mycoses Subsets: MEDLINE

Original Publication: Berlin : Grosse Verlag, [1988?-

Antigens, Fungal/*analysis ; Cryptococcus/*isolation & purification ; Meningitis, Cryptococcal/*diagnosis ; Molecular Diagnostic Techniques/*methods; Adult ; Antifungal Agents/therapeutic use ; Antigens, Fungal/genetics ; Carbon ; Cryptococcus/genetics ; Cryptococcus/growth & development ; DNA, Fungal/isolation & purification ; Female ; Humans ; Immunocompromised Host ; Male ; Meningitis, Cryptococcal/cerebrospinal fluid ; Meningitis, Cryptococcal/microbiology ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity

Cryptococcal meningitis (CM) is a life-threatening mycosis primarily occurring in HIV-infected individuals. Recently, non-HIV-infected hosts were increasingly reported to form a considerable proportion. However, the majority of the reported studies on the diagnosis of CM patients were performed on HIV-infected patients. For evaluation of various diagnostic approaches for CM in non-HIV-infected patients, a range of conventional and molecular assays used for diagnosis of CM were verified on 85 clinical CSFs from non-HIV-infected CM patients, including India ink staining, culture, a newly developed loop-mediated isothermal amplification (LAMP), the lateral flow assay (LFA) of cryptococcal antigen detection and a qPCR assay. The LFA had the highest positive detection rate (97.6%; 95% CI, 91.8-99.7%) in non-HIV-infected CM patients, followed by the LAMP (87.1%; 95% CI, 78.0-93.4%), the qPCR (80.0%; 95% CI, 69.9-87.9%), India ink staining (70.6%; 95% CI, 59.7-80.0%) and culture (35.3%; 95% CI, 25.2-46.4%). All culture positive specimens were correctly identified by the LFA.
(© 2016 Blackwell Verlag GmbH.)

Keywords: Cryptococcal meningitis; Diagnosis; non-HIV-infected host

GENBANK 2013SMMU-LL013; EF211264

0 (Antifungal Agents)
0 (Antigens, Fungal)
0 (DNA, Fungal)
0 (chinese ink)
7440-44-0 (Carbon)

Date Created: 20160411 Date Completed: 20170321 Latest Revision: 20191210

20250114

10.1111/myc.12497

27061343