Membrane proteins involved in transport, vesicle traffic and Ca(2+) signaling increase in beetroots grown in saline soils.

Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag [etc.]

Beta vulgaris/*metabolism ; Calcium/*metabolism ; Membrane Proteins/*metabolism ; Plant Proteins/*metabolism ; Plant Roots/*metabolism ; Soil/*chemistry; Beta vulgaris/growth & development ; Biological Transport ; Cell Membrane/metabolism ; Electrophoresis, Polyacrylamide Gel ; Hydrophobic and Hydrophilic Interactions ; Mass Spectrometry/methods ; Membrane Proteins/chemistry ; Membrane Proteins/isolation & purification ; Plant Proteins/chemistry ; Plant Proteins/isolation & purification ; Plant Roots/growth & development ; Sodium Chloride/chemistry ; Transport Vesicles/metabolism ; Vacuolar Proton-Translocating ATPases/chemistry ; Vacuolar Proton-Translocating ATPases/isolation & purification ; Vacuolar Proton-Translocating ATPases/metabolism

Main Conclusion: By separating plasma membrane proteins according to their hydropathy from beetroots grown in saline soils, several proteins probably involved in salt tolerance were identified by mass spectrometry. Beetroots, as a salt-tolerant crop, have developed mechanisms to cope with stresses associated with saline soils. To observe which plasma membrane (PM) proteins were more abundant in beet roots grown in saline soils, beet root plants were irrigated with water or 0.2 M NaCl. PM-enriched membrane preparations were obtained from these plants, and their proteins were separated according to their hydropathy by serial phase partitioning with Triton X-114. Some proteins whose abundance increased visibly in membranes from salt-grown beetroots were identified by mass spectrometry. Among them, there was a V-type H(+)-ATPase (probably from contaminating vacuolar membranes), which increased with salt at all stages of beetroots' development. Proteins involved in solute transport (an H(+)-transporting PPase and annexins), vesicle traffic (clathrin and synaptotagmins), signal perception and transduction (protein kinases and phospholipases, mostly involved in calcium signaling) and metabolism, appeared to increase in salt-grown beetroot PM-enriched membranes. These results suggest that PM and vacuolar proteins involved in transport, metabolism and signal transduction increase in beet roots adapted to saline soils. In addition, these results show that serial phase partitioning with Triton X-114 is a useful method to separate membrane proteins for their identification by mass spectrometry.

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Keywords: Beta vulgaris; Mass spectrometry; Plasma membrane proteins; Salt tolerance; Triton X-114

0 (Membrane Proteins)
0 (Plant Proteins)
0 (Soil)
451W47IQ8X (Sodium Chloride)
EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SY7Q814VUP (Calcium)

Date Created: 20160313 Date Completed: 20170410 Latest Revision: 20181113

20231215

10.1007/s00425-016-2488-4

26969021